Kishi M, Arimura Y, Ikuta K, Shoya Y, Lai P K, Kakinuma M
Section of Serology, Hokkaido University, Sapporo, Japan.
J Virol. 1996 Jan;70(1):635-40. doi: 10.1128/JVI.70.1.635-640.1996.
A cDNA fragment of the Borna disease virus (BDV) open reading frame II (ORF-II), which encodes a 24-kDa phosphoprotein (p24 [P protein]), was amplified from total RNA of peripheral blood mononuclear cells (PBMC) from three psychiatric inpatients. The amplified cDNA fragments were cloned, sequenced, and analyzed. A total of 15 clones, 5 from each patient, were studied. Intrapatient divergencies of the BDV ORF-II nucleotide sequence were 4.2 to 7.3%, 4.8 to 7.3%, and 2.8 to 7.1% for the three patients, leading to differences of 7.7 to 14.5%, 10.3 to 17.1%, and 6.0 to 16.2%, respectively, in the deduced amino acid sequence for BDV p24. Interpatient divergencies among the 15 clones were 5.9 to 12.7% at the nucleotide level and 12.8 to 28.2% at the amino acid level. Thus, in p24, BDV in human PBMC of the patients undergoes mutation at high rates in vivo. Additionally, we found that the nucleotide sequence of the 15 human BDV ORF-II cDNA clones differed from those of the horse strains V and He/80-1 by 4.2 to 9.3%. However, comparison of the consensus amino acid sequence deduced from the 15 human clones with those of the horse strains revealed no human-specific amino acid residue, suggesting that the BDV infecting humans may be related to that infecting horses.
从三名精神科住院患者的外周血单个核细胞（PBMC）的总RNA中扩增出博尔纳病病毒（BDV）开放阅读框II（ORF-II）的cDNA片段，该片段编码一种24 kDa的磷蛋白（p24 [P蛋白]）。对扩增出的cDNA片段进行克隆、测序和分析。共研究了15个克隆，每个患者5个。三名患者的BDV ORF-II核苷酸序列的患者内差异分别为4.2%至7.3%、4.8%至7.3%和2.8%至7.1%，导致BDV p24推导氨基酸序列的差异分别为7.7%至14.5%、10.3%至17.1%和6.0%至16.2%。15个克隆之间的患者间差异在核苷酸水平为5.9%至12.7%，在氨基酸水平为12.8%至28.2%。因此，在患者的人PBMC中的BDV的p24在体内以高频率发生突变。此外，我们发现15个人BDV ORF-II cDNA克隆的核苷酸序列与马毒株V和He/80-1的核苷酸序列相差4.2%至9.3%。然而，将从15个人克隆推导的共有氨基酸序列与马毒株的序列进行比较，未发现人特异性氨基酸残基，这表明感染人类的BDV可能与感染马的BDV有关。
