de Graeff-Meeder E R, Rijkers G T, Voorhorst-Ogink M M, Kuis W, van der Zee R, van Eden W, Zegers B J
Department of Immunology, University Hospital for Children and Youth Het Wilhelmina Kinderziekenhuis, Utrecht, The Netherlands.
Pediatr Res. 1993 Oct;34(4):424-8. doi: 10.1203/00006450-199310000-00008.
The 60-kD heat shock protein (hsp60) has been implicated in the etiology and pathogenesis of both experimental and naturally occurring autoimmune diseases such as juvenile chronic arthritis (JCA). Human hsp60 is expressed in inflamed synovial tissue, and T lymphocytes both from peripheral blood and synovial fluid show reactivity to human hsp60. Because the anti-hsp60 B lymphocyte response has been less well studied, we have determined the occurrence of IgG anti-human hsp60 antibodies in patients with JCA and various other autoimmune diseases of childhood. Serum IgG anti-human hsp60 antibodies in JCA patients were significantly higher compared with control children (358 and 163 U/mL, respectively). Within the group of JCA patients, the highest antibody titers were found in the subgroup with a polyarticular onset of JCA. IgG anti-human hsp60 antibody levels in synovial fluid were 3- to 4-fold higher compared with paired serum samples. Because this difference was not found for total IgG or for irrelevant antibodies (anti-polyribosylribitol phosphate), this suggests local anti-hsp60 antibody production in the synovial compartment. The occurrence of anti-hsp60 antibodies is not specific for JCA but also is found in children with systemic lupus erythematosus and in cystic fibrosis, whereas mixed connective tissue disease and insulin-dependent diabetes are negative in this respect. Whether the anti-human hsp60 antibodies are directed toward species-specific sequences or to conserved sequences of the hsp60 molecule remains to be determined.
60kD热休克蛋白（hsp60）与实验性和自然发生的自身免疫性疾病（如青少年慢性关节炎，JCA）的病因和发病机制有关。人hsp60在炎症滑膜组织中表达，外周血和滑液中的T淋巴细胞均对人hsp60有反应性。由于抗hsp60 B淋巴细胞反应的研究较少，我们测定了JCA患者以及其他各种儿童自身免疫性疾病患者中IgG抗人hsp60抗体的发生情况。与对照儿童相比，JCA患者血清中的IgG抗人hsp60抗体显著更高（分别为358和163 U/mL）。在JCA患者组中，多关节起病的JCA亚组抗体滴度最高。滑液中IgG抗人hsp60抗体水平比配对血清样本高3至4倍。由于总IgG或无关抗体（抗聚核糖醇磷酸）未发现这种差异，这表明滑膜腔室中有局部抗hsp60抗体产生。抗hsp60抗体的出现并非JCA所特有，在系统性红斑狼疮患儿和囊性纤维化患儿中也可发现，而混合性结缔组织病和胰岛素依赖型糖尿病患儿在这方面呈阴性。抗人hsp60抗体是针对hsp60分子的种属特异性序列还是保守序列仍有待确定。
