Van Heusden G P, de Koning W J, van der Aart Q J, van den Berg J A, Steensma H Y
Department of Molecular and Cellular Biology, Leiden University, Clusius Laboratory, The Netherlands.
Yeast. 1993 Oct;9(10):1107-10. doi: 10.1002/yea.320091011.
The nucleotide sequence was determined of a 2.1 kb DNA fragment located at approximately 35 kb to the right of the centromere of chromosome VI from Saccharomyces cerevisiae. Analysis revealed the presence of a tRNA(GLy) gene, part of a delta element and a remarkable palindromic sequence. The longest open reading frame found encodes a putative protein of 195 amino acids. Although the fragment was isolated by hybridization to a human diacylglycerol kinase cDNA, no evidence was obtained for the presence of a gene encoding diacylglycerol kinase.
测定了来自酿酒酵母第六条染色体着丝粒右侧约35 kb处一个2.1 kb DNA片段的核苷酸序列。分析显示存在一个tRNA(Gly)基因、一个δ元件的一部分以及一个显著的回文序列。所发现的最长开放阅读框编码一个含195个氨基酸的推定蛋白。尽管该片段是通过与人二酰基甘油激酶cDNA杂交分离得到的,但未获得存在编码二酰基甘油激酶基因的证据。