Threlkeld A B, Froggatt J W, Schein O D, Forman M S
Wilmer Ophthamological Institute, Johns Hopkins University and Hospital, Baltimore, MD.
Ophthalmology. 1993 Dec;100(12):1841-5. doi: 10.1016/s0161-6420(93)31388-6.
To determine the efficacy of a disinfectant wipe method in eliminating adenovirus 8 from both Goldmann tonometer and pneumotonometer tips, and to determine the efficacy of 5-minute disinfectant soaks in removing the virus from Goldmann tonometer tips.
Quantification of adenovirus 8 in the ocular secretions of six infected patients was performed. Clinically relevant inocula of adenovirus 8 were then prepared in a serum suspension which was used to contaminate Goldmann tonometer and pneumotonometer tips. Each contaminated tip was subjected to either a dry wipe or a wipe with one of the following: water, isopropyl alcohol, hydrogen peroxide, and iodophor. The tips were than quantitatively assayed for residual adenovirus 8. Disinfectant soaks were similarly assessed using contaminated Goldmann tonometer tips soaked in water, hydrogen peroxide, iodophor, and sodium hypochlorite.
Adenovirus 8 was recovered from both types of tonometer tips after dry wipes, but no viable virus was recovered from tips wiped with water only or with any of the disinfectants tested. Adenovirus 8 also was recovered from Goldmann tonometer tips that were either rinsed or soaked in water; no virus was recovered from tips soaked for 5 minutes in any of the disinfectants tested.
A disinfectant wipe method using isopropyl alcohol, hydrogen peroxide, or iodophor is effective in removing adenovirus 8 from both Goldmann tonometer and pneumotonometer tips. Five-minute disinfectant soaks also are effective for Goldmann tonometer tips.
确定消毒擦拭法从戈德曼眼压计和气动眼压计尖端清除8型腺病毒的效果，并确定5分钟消毒剂浸泡法从戈德曼眼压计尖端去除病毒的效果。
对6名感染患者的眼分泌物中的8型腺病毒进行定量分析。然后在血清悬液中制备具有临床相关性的8型腺病毒接种物，用于污染戈德曼眼压计和气动眼压计尖端。每个受污染的尖端进行干擦或用以下之一擦拭：水、异丙醇、过氧化氢和碘伏。然后对尖端进行8型腺病毒残留量的定量检测。使用浸泡在水、过氧化氢、碘伏和次氯酸钠中的受污染戈德曼眼压计尖端对消毒剂浸泡法进行类似评估。
干擦后两种眼压计尖端均检测到8型腺病毒，但仅用水或任何一种测试消毒剂擦拭的尖端未检测到活病毒。冲洗或浸泡在水中的戈德曼眼压计尖端也检测到8型腺病毒；在任何一种测试消毒剂中浸泡5分钟的尖端未检测到病毒。
使用异丙醇、过氧化氢或碘伏的消毒擦拭法可有效从戈德曼眼压计和气动眼压计尖端清除8型腺病毒。5分钟消毒剂浸泡法对戈德曼眼压计尖端也有效。
