Segal W A, Pirnazar J R, Arens M, Pepose J S
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri, USA
Am J Ophthalmol. 2001 Feb;131(2):184-7. doi: 10.1016/s0002-9394(00)00784-4.
To compare methods of disinfecting Goldmann tonometer tips inoculated with hepatitis C virus.
Hepatitis C virus was placed on Goldmann tonometer tips, air dried, and then disinfected by dry gauze wipes, isopropyl alcohol wipes, cold water washes, povidone iodine 10% wipes, and hydrogen peroxide or isopropyl alcohol soaks followed by a cold water wash and dry. Hydrogen peroxide and isopropyl alcohol disinfection techniques followed the Centers for Disease Control and Prevention guidelines for prevention of possible transmission of human immunodeficiency virus (HIV). After disinfection, samples from tonometer tips were amplified by polymerase chain reaction to quantitate the amount of hepatitis C virus RNA remaining.
Percentage of hepatitis C virus RNA remaining after disinfection: dry gauze wipes 95.65%, isopropyl alcohol 5-second wipes 88.91%, cold water wash 4.78%, povidone iodine 10% 5-second wipes 0.72%, hydrogen peroxide soak with cold water wash 0.07%, and isopropyl alcohol soak and cold water wash 0.02%.
After inoculation of Goldmann tonometer tips with hepatitis C virus, a 5-minute soak in 3% hydrogen peroxide or 70% isopropyl alcohol followed by washing in cold water resulted in the greatest reduction in hepatitis C virus RNA.
比较对接种丙型肝炎病毒的戈德曼眼压计探头进行消毒的方法。
将丙型肝炎病毒置于戈德曼眼压计探头上，自然风干，然后分别用干纱布擦拭、异丙醇擦拭、冷水冲洗、10%聚维酮碘擦拭，以及用过氧化氢或异丙醇浸泡后再用冷水冲洗并干燥等方法进行消毒。过氧化氢和异丙醇消毒技术遵循美国疾病控制与预防中心预防人类免疫缺陷病毒（HIV）可能传播的指南。消毒后，通过聚合酶链反应对眼压计探头的样本进行扩增，以定量残留的丙型肝炎病毒RNA的量。
消毒后残留丙型肝炎病毒RNA的百分比：干纱布擦拭为95.65%，异丙醇5秒擦拭为88.91%，冷水冲洗为4.78%，10%聚维酮碘5秒擦拭为0.72%，过氧化氢浸泡后冷水冲洗为0.07%，异丙醇浸泡后冷水冲洗为0.02%。
用丙型肝炎病毒接种戈德曼眼压计探头后，在3%过氧化氢或70%异丙醇中浸泡5分钟，然后用冷水冲洗，可使丙型肝炎病毒RNA的减少量最大。
