乳胶凝集试验与酶联免疫吸附测定法检测水痘-带状疱疹病毒抗体的比较。
Comparison of latex agglutination test with enzyme-linked immunosorbent assay for detection of antibody to varicella-zoster virus.
作者信息
Landry M L, Ferguson D
机构信息
Clinical Virology Laboratory, Yale-New Haven Hospital, Connecticut.
出版信息
J Clin Microbiol. 1993 Nov;31(11):3031-3. doi: 10.1128/jcm.31.11.3031-3033.1993.
A new latex agglutination (LA) test (VZVscan; Becton Dickinson, Cockeysville, Md.) was compared with an enzyme-linked immunosorbent assay (ELISA) (Varicella STAT; Whittaker Bioproducts, Walkersville, Md.) for detection of varicella-zoster virus (VZV) antibody. Of 165 samples tested, 126 (76%) were positive by LA, 123 (73%) were positive by ELISA, and 35 (21%) were negative by both methods. Six samples (4%) were LA positive and ELISA negative or equivocal; three samples (2%) were ELISA positive and LA negative. However, LA failed to detect seroconversions in four adults with varicella in samples obtained 14 to 17 days after the onset of rash. These same samples had ELISA values in the high-positive range. In addition, the recommended LA screening dilution (1:2) is not supported by published data and should be changed.
将一种新的乳胶凝集试验（LA）（VZVscan；Becton Dickinson公司，科基斯维尔，马里兰州）与酶联免疫吸附测定（ELISA）（水痘STAT；惠特克生物制品公司，沃克斯维尔，马里兰州）进行比较，以检测水痘带状疱疹病毒（VZV）抗体。在检测的165份样本中，126份（76%）通过LA检测为阳性，123份（73%）通过ELISA检测为阳性，35份（21%）两种方法检测均为阴性。6份样本（4%）LA检测为阳性而ELISA检测为阴性或不确定；3份样本（2%）ELISA检测为阳性而LA检测为阴性。然而，LA未能在4名患水痘的成年人出疹后14至17天采集的样本中检测到血清转化。这些相同的样本ELISA值处于高阳性范围。此外，已发表的数据不支持推荐的LA筛查稀释度（1:2），应予以更改。
Zotero 插件