Bollensen E, Albrecht S, Beuche W, Mäder M, Prange H W
Department of Neurology, University of Göttingen, Germany.
J Neurol. 1993 Sep;240(8):471-4. doi: 10.1007/BF00874115.
The reactivity and specificity of locally produced cerebrospinal fluid (CSF) antibodies against antigens of Treponema pallidum were assessed by Western blotting in patients with clinical signs of parenchymal or meningovascular neurosyphilis. All nine patients showed local production of treponeme-specific antibodies in the central nervous system (CNS). In most of the patients serum and CSF antibodies were bound to the same antigens: the common treponemal 48/45 kDa protein and the putative specific T. pallidum protein in the range of 12-14 kDa. In some patients the intensity of staining obtained by CSF antibodies was higher than that derived from serum, indicating locally produced antibodies. In contrast to other more acute inflammatory CNS diseases, no expanded or different antigen binding of the CSF antibodies compared with serum antibodies was found in neurosyphilic patients. The results presented are discussed with regard to the role of the blood-brain barrier in antibody concentrations of CSF and serum.
通过蛋白质印迹法评估了患有实质性或脑膜血管性神经梅毒临床症状患者中局部产生的脑脊液（CSF）抗体针对梅毒螺旋体抗原的反应性和特异性。所有9例患者均显示中枢神经系统（CNS）中局部产生梅毒螺旋体特异性抗体。大多数患者的血清和脑脊液抗体与相同抗原结合：常见的梅毒螺旋体48/45 kDa蛋白以及12 - 14 kDa范围内假定的梅毒螺旋体特异性蛋白。在一些患者中，脑脊液抗体获得的染色强度高于血清抗体，表明存在局部产生的抗体。与其他更急性的炎症性中枢神经系统疾病不同，在神经梅毒患者中未发现脑脊液抗体与血清抗体相比有扩大或不同的抗原结合。结合血脑屏障在脑脊液和血清抗体浓度中的作用对所呈现的结果进行了讨论。
