果蝇的核糖体。III. 黑腹果蝇和拟暗果蝇之间的RNA与蛋白质同源性。
The ribosomes of Drosophila. III. RNA and protein homology between D. melanogaster and D. virilis.
作者信息
Weber L, Berger E, Vaslet C, Yedvobnick B
出版信息
Genetics. 1976 Nov;84(3):573-85. doi: 10.1093/genetics/84.3.573.
Abstract
The extent of interspecific homology between D. melanogaster and D. virilis for ribosomal RNA and ribosomal protein was examined using the techniques of two-dimensional gel electrophoresis, and RNA-DNA filter hybridization. Only 2 of the 71 ribosomal proteins resolved were found to be species specific, while comparisons of soluble larval hemolymph protein patterns showed little similarity. Depending on the technique employed, the sequence homology for 18S + 28S ribosomal RNA was found to be between 83-94%, and sequence homology for 5S rRNA was judged to be complete.
摘要
利用二维凝胶电泳技术和RNA-DNA滤膜杂交技术，研究了黑腹果蝇和粗壮果蝇核糖体RNA及核糖体蛋白的种间同源程度。在解析出的71种核糖体蛋白中，只有2种是物种特异性的，而对可溶性幼虫血淋巴蛋白模式的比较显示相似度很低。根据所采用的技术，发现18S + 28S核糖体RNA的序列同源性在83%至94%之间，5S rRNA的序列同源性被判定为完全相同。
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