Richman A V
J Natl Cancer Inst. 1976 Aug;57(2):263-7. doi: 10.1093/jnci/57.2.263.
Indirect immunofluorescence studies on sections of human normal breast tissue, benign breast lesions, and breast adenocarcinomas were performed with the use of monospecific fluorescein-conjugated antisera to human alpha, gamma, and mu chains, albumin, and C3. In all tissues, benign and malignant, immunohistologic localization of IgA and IgM was noted in patterns previously described for other secretory epithelia. IgG was localized at the tumor cell, but was not associated with epithelial cells from either normal breast tissue or benign breast lesions. Except for a single case of benign cystosarcoma phylloides, all normal, benign, and malignant breast tissues failed to react with anti-C3. These findings were compared with previous studies employing dissociated tumor cells, and the potential application of these methods to pathologic diagnosis was examined.
利用与人类α、γ和μ链、白蛋白及C3的单特异性荧光素结合抗血清，对人正常乳腺组织、乳腺良性病变及乳腺腺癌切片进行了间接免疫荧光研究。在所有良性和恶性组织中，IgA和IgM的免疫组织学定位呈先前描述的其他分泌上皮的模式。IgG定位于肿瘤细胞，但与正常乳腺组织或乳腺良性病变的上皮细胞无关。除1例良性叶状囊肉瘤外，所有正常、良性和恶性乳腺组织均未与抗C3发生反应。将这些发现与先前使用解离肿瘤细胞的研究进行了比较，并探讨了这些方法在病理诊断中的潜在应用。
