Dollar M L, Sly M K, Credi R G, Constantinescu A, Tsai C C, Kulkarni P V, Clagett G P, Eberhart R C
Biomedical Engineering Program, University of Texas Southwestern Medical Center at Dallas 75235-9031.
ASAIO J. 1993 Jul-Sep;39(3):M268-72.
An important element in the evaluation of biomaterials is quantification of the relationships and the sequence of events between blood elements, blood flow, and the foreign surface. We adapted a qualitative two-dimensional 111In-labeled platelet imaging method to a quantitative noninvasive analysis of platelet uptake/release kinetics for infusion catheters in a canine model. Bilateral femoral vein 6 Fr. Groshong catheters (one treated with a hydroxylated siloxane to improve albumin affinity) were monitored at femoral vein sites with a GE 400T gamma camera, interfaced with a Technicare 560 image acquisition computer. The field of view was sufficiently large that all events below the diaphragm were recorded without having to move the camera. Image acquisition time was 2.5 min; images were obtained every 5-15 min for 3 hrs. Continuous recordings were obtained from bilateral ultrasonic velocity probes, attached distal to the catheter implant sites. A 5 ml blood sample was placed in the field to permit calibration of gamma emissions per pixel in terms of labeled platelet density. Signal compensation for near field capillary perfusion was performed. The two-dimensional platelet distribution was computed and displayed. Local, time dependent platelet accumulation on the catheters and adjacent vessel walls was observed. Platelet accumulation proceeded in irregular steps during the implant period. Loss of local platelet deposits was observed. Downstream reattachment of platelet emboli was inferred from simultaneous reductions and increases in local platelet densities at two catheter positions. Platelet attachment was inversely related to vein blood velocity.(ABSTRACT TRUNCATED AT 250 WORDS)
生物材料评估中的一个重要因素是对血液成分、血流和异物表面之间关系及事件顺序进行量化。我们将定性二维铟 - 111标记血小板成像方法应用于犬模型中输注导管血小板摄取/释放动力学的定量无创分析。双侧股静脉置入6F Groshong导管（其中一根用羟基化硅氧烷处理以提高白蛋白亲和力），通过GE 400Tγ相机在股静脉部位进行监测，并与Technicare 560图像采集计算机相连。视野足够大，无需移动相机即可记录横膈膜以下的所有事件。图像采集时间为2.5分钟；每5 - 15分钟采集一次图像，持续3小时。从附着在导管植入部位远端的双侧超声速度探头获取连续记录。将5毫升血液样本置于视野中，以便根据标记血小板密度对每个像素的γ射线发射进行校准。对近场毛细血管灌注进行信号补偿。计算并显示二维血小板分布。观察到导管和相邻血管壁上局部血小板随时间的积累情况。在植入期间，血小板积累呈不规则步骤进行。观察到局部血小板沉积物的减少。从两个导管位置局部血小板密度的同时降低和增加推断出血小板栓子在下游的重新附着。血小板附着与静脉血流速度呈负相关。（摘要截断于250字）
