Müllbacher A
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra City ACT.
J Exp Med. 1994 Jan 1;179(1):317-21. doi: 10.1084/jem.179.1.317.
I have used the transfer of primed lymphocytes into syngeneic irradiated recipients to investigate whether the persistence of antigen is required in the long-term maintenance of cytolytic T cell memory to influenza virus. Animals were immunized with influenza virus (A/WSN) and used 17 wk later as either donors for T cells or as lethally irradiated recipients. Naive age-matched mice served as controls. At intervals of 4, 8, 16, and 25 wk after T cell transfer, experimental and control groups were immunized with a heterologous virus (A/JAP) and splenocytes tested for lytic activity to influenza virus 3 and 6 d after immunization. Lytic activity 3 d after infection (a property exclusive to a memory cytotoxic T cell response) (Effros, R. B., J. Bennink, and P. C. Doherty. 1978. Cell. Immunol. 36:345.; and Hill, A. B., R. V. Blanden, C. R. Parrish, and A. Müllbacher. 1992. Immunol. Cell Biol. 70:259), was only observed by primed and naive irradiated recipients reconstituted with memory T cells. No day 3 responses were observed when naive T cells were transferred into irradiated primed or unprimed recipients. These observations demonstrate that cytolytic T cell memory to influenza virus is long lived in the absence of antigen.
我采用将致敏淋巴细胞转移至同基因受照射受体的方法，来研究在对流感病毒的细胞毒性T细胞记忆的长期维持中，抗原的持续存在是否是必需的。用流感病毒（A/WSN）免疫动物，17周后将其用作T细胞供体或致死剂量照射的受体。年龄匹配的未免疫小鼠作为对照。在T细胞转移后的4、8、16和25周时，对实验组和对照组用异源病毒（A/JAP）进行免疫，并在免疫后3天和6天检测脾细胞对流感病毒的裂解活性。感染后3天的裂解活性（这是记忆性细胞毒性T细胞反应特有的特性）（埃弗罗斯，R.B.，J.本宁克，和P.C.多尔蒂。1978年。《细胞免疫学》36:345；以及希尔，A.B.，R.V.布兰登，C.R.帕里什，和A.米尔巴赫。1992年。《免疫与细胞生物学》70:259），仅在接受记忆T细胞重建的致敏和未致敏受照射受体中观察到。当将未致敏T细胞转移至受照射的致敏或未致敏受体时，未观察到第3天的反应。这些观察结果表明，在没有抗原的情况下，对流感病毒的细胞毒性T细胞记忆可以长期存在。
