来自表皮葡萄球菌的肽聚糖和磷壁酸刺激人单核细胞释放肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6。
Peptidoglycan and teichoic acid from Staphylococcus epidermidis stimulate human monocytes to release tumour necrosis factor-alpha, interleukin-1 beta and interleukin-6.
作者信息
Mattsson E, Verhage L, Rollof J, Fleer A, Verhoef J, van Dijk H
机构信息
Eijkman-Winkler Institute of Medical and Clinical Microbiology, Utrecht University, The Netherlands.
出版信息
FEMS Immunol Med Microbiol. 1993 Oct;7(3):281-7. doi: 10.1111/j.1574-695X.1993.tb00409.x.
Cytokines play a major role in the pathophysiology of septic shock. In this study, human peripheral blood monocytes were stimulated with peptidoglycan and teichoic acid, purified from a strain of Staphylococcus epidermidis. Polymyxin B (PM-B) was added to avoid the effects of possible contamination with endotoxin. Tumour necrosis factor-alpha (TNF), interleukin-1 beta (IL-1), and interleukin-6 (IL-6) in the supernates were measured by enzyme-linked immunosorbent assays. Peptidoglycan and teichoic acid induced TNF, IL-1, and IL-6 in a concentration-dependent manner. Teichoic acid was a weaker inducer than peptidoglycan, especially for IL-1. Lipopolysaccharide from an E. coli strain was used as a control, being 100-1000 times more potent than peptidoglycan and teichoic acid.
细胞因子在脓毒性休克的病理生理学中起主要作用。在本研究中，用人外周血单核细胞与从表皮葡萄球菌菌株中纯化的肽聚糖和磷壁酸进行刺激。加入多粘菌素B（PM-B）以避免内毒素污染可能产生的影响。通过酶联免疫吸附测定法测量上清液中的肿瘤坏死因子-α（TNF）、白细胞介素-1β（IL-1）和白细胞介素-6（IL-6）。肽聚糖和磷壁酸以浓度依赖性方式诱导TNF、IL-1和IL-6。磷壁酸作为诱导剂比肽聚糖弱，尤其是对IL-1而言。来自大肠杆菌菌株的脂多糖用作对照，其效力比肽聚糖和磷壁酸强100 - 1000倍。
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