Keith C S, Hoang D O, Barrett B M, Feigelman B, Nelson M C, Thai H, Baysdorfer C
Department of Biological Sciences, California State University, Hayward 94542.
Plant Physiol. 1993 Jan;101(1):329-32. doi: 10.1104/pp.101.1.329.
As part of a project to identify novel maize (Zea mays L. cv B73) genes functionally, we have partially sequenced 130 randomly selected clones from a maize leaf cDNA library. Data base comparisons revealed seven previously sequenced maize cDNAs and 18 cDNAs with sequence similarity to related maize genes or to genes from other organisms. One hundred five cDNAs show little or no similarity to previously sequenced genes. Our results also establish the suitability of this library for large-scale sequencing in terms of its large insert size, proper insert orientation, and low duplication rate.
作为一项功能鉴定玉米(Zea mays L. cv B73)新基因的项目的一部分,我们对从玉米叶片cDNA文库中随机挑选的130个克隆进行了部分测序。数据库比对揭示了7个先前已测序的玉米cDNA以及18个与相关玉米基因或其他生物体基因具有序列相似性的cDNA。105个cDNA与先前测序的基因几乎没有或没有相似性。我们的结果还表明,就其大插入片段大小、正确的插入方向和低重复率而言,该文库适合大规模测序。