Viladot J L, Stone B, Driguez H, Planas A
Laboratori de Bioquímica, Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.
Carbohydr Res. 1998 Sep;311(1-2):95-9. doi: 10.1016/s0008-6215(98)00212-2.
Enzymatic hydrolysis of barley (1-->3),(1-->4)-beta-D-glucan using a recombinant (1-->3),(1-->4)-beta-glucanase from Bacillus licheniformis gives Glc beta 4Glc beta 3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding beta-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glc beta 4Glc beta 3Glc beta 4Glc beta 4Glc beta 3Glc in 20% yield.
使用地衣芽孢杆菌的重组（1→3），（1→4）-β-葡聚糖酶对大麦（1→3），（1→4）-β-D-葡聚糖进行酶促水解，乙酰化后分离得到的Glcβ4Glcβ3Glc产率为49%。传统处理产生了相应的β-氟化物，并对其进行了仔细的脱O-乙酰化。由标题酶催化的该底物的转糖基化反应，以20%的产率得到了Glcβ4Glcβ3Glcβ4Glcβ4Glcβ3Glc。
