Joó F
Laboratory of Molecular Neurobiology, Biological Research Center, Szeged, Hungary.
Neurochem Int. 1993 Dec;23(6):499-521. doi: 10.1016/0197-0186(93)90098-p.
Ever since the discovery of Paul Ehrlich (1885 Das Sauerstoff-bedürfnis des Organismus: Hirschwald, Berlin) about the restricted material exchange, existing between the blood and the brain, the ultimate goal of subsequent studies has been mainly directed towards the elucidation of relative importance of different cellular compartments in the peculiar penetration barrier consisting the structural basis of the blood-brain barrier (BBB). It is now generally agreed that, in most vertebrates, the endothelial cells of the central nervous system (CNS) are responsible for the unique penetration barrier, which restricts the free passage of nutrients, hormones, immunologically relevant molecules and drugs to the brain. After an era of studying with endogenous or exogenous tracers the unique permeability properties of cerebral endothelial cells in vivo, the next generation, i.e. the in vitro blood-brain barrier model system was introduced in 1973. Recent advances in our knowledge of the BBB have in part been made by studying the properties and function of cerebral endothelial cells (CEC) with this in vitro approach. This review summarizes the results obtained on isolated brain microvessels in the second decade of its advent.
自从保罗·埃尔利希（1885年《有机体的氧气需求：希尔施瓦尔德，柏林》）发现血液与大脑之间存在有限的物质交换以来，后续研究的最终目标主要是阐明构成血脑屏障（BBB）结构基础的特殊渗透屏障中不同细胞成分的相对重要性。现在人们普遍认为，在大多数脊椎动物中，中枢神经系统（CNS）的内皮细胞负责这种独特的渗透屏障，它限制了营养物质、激素、免疫相关分子和药物进入大脑的自由通道。在经历了用内源性或外源性示踪剂研究体内脑内皮细胞独特通透性特性的时代后，1973年引入了下一代，即体外血脑屏障模型系统。我们对血脑屏障认识的最新进展部分得益于用这种体外方法研究脑内皮细胞（CEC）的特性和功能。这篇综述总结了在其出现后的第二个十年里，在分离的脑微血管上所获得的结果。
