Schmalzing D, Piggee C A, Foret F, Carrilho E, Karger B L
Northeastern University, Barnett Institute, Boston, MA 02115.
J Chromatogr A. 1993 Oct 15;652(1):149-59. doi: 10.1016/0021-9673(93)80655-R.
Polyvinylmethylsiloxanediol (50% vinyl) was synthesized and combined with a cross-linker for static coating onto fused-silica columns. After cross-linking and binding to the surface, linear polyacrylamide was grafted to the double bonds of the siloxanediol; subsequently, this linear polymer matrix was cross-linked with formaldehyde. The grafted neutral polymeric layer provided suppression of electroosmotic flow and minimized adsorption. This combination yielded successful open tube and polymer network separations of proteins, peptides and DNA molecules. Very high efficiencies (ca. 1 x 10(6) plates/m) were achieved for open tube protein separations, and hundreds of consecutive runs were performed with minimal change in migration times.
合成了乙烯基甲基硅氧烷二醇（50%乙烯基），并与一种交联剂混合，用于在熔融石英柱上进行静态涂覆。交联并结合到表面后，将线性聚丙烯酰胺接枝到硅氧烷二醇的双键上；随后，该线性聚合物基质与甲醛交联。接枝的中性聚合物层抑制了电渗流，并使吸附最小化。这种组合成功实现了对蛋白质、肽和DNA分子的开管和聚合物网络分离。开管蛋白质分离实现了非常高的效率（约1×10⁶塔板数/米），并且在迁移时间变化最小的情况下进行了数百次连续运行。
