• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用DMPO（5,5 - 二甲基吡咯啉 - N - 氧化物）对超氧自由基和羟基自由基进行自旋捕捉：更多关于铁的研究

The spin trapping of superoxide and hydroxyl free radicals with DMPO (5,5-dimethylpyrroline-N-oxide): more about iron.

作者信息

Buettner G R

机构信息

ESR Facility/EMRB 68, College of Medicine, University of Iowa, Iowa City 52242.

出版信息

Free Radic Res Commun. 1993;19 Suppl 1(0 1):S79-87. doi: 10.3109/10715769309056s79.

DOI:10.3109/10715769309056s79
PMID:8282235
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4669197/
Abstract

The reaction of superoxide with the spin trap DMPO (5,5-dimethylpyrroline-N-oxide) is widely used to study superoxide production as well as issues associated with the superoxide-related formation of hydroxyl radical. However, the interpretation of observed intensities of DMPO/.OOH and DMPO/.OH signals in electron paramagnetic resonance spin trapping experiments is not without its difficulties. In this paper, I report experiments that demonstrate: 1. That the flux of superoxide formation in a DMPO spin trapping experiment can alter the apparent importance of weak DMPO/.OH signals; 2. That iron can influence the DMPO/.OOH spin trapping results; 3. That there is very little spontaneous breakdown of DMPO/.OOH to form DMPO/.OH.

摘要

超氧化物与自旋捕捉剂DMPO（5,5 - 二甲基吡咯啉 - N - 氧化物）的反应被广泛用于研究超氧化物的产生以及与超氧化物相关的羟基自由基形成的相关问题。然而，在电子顺磁共振自旋捕捉实验中，对观察到的DMPO/.OOH和DMPO/.OH信号强度的解释并非没有困难。在本文中，我报告的实验表明：1. 在DMPO自旋捕捉实验中超氧化物形成的通量可以改变微弱的DMPO/.OH信号的表观重要性；2. 铁可以影响DMPO/.OOH自旋捕捉结果；3. DMPO/.OOH自发分解形成DMPO/.OH的情况非常少。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/f332f4591d86/nihms736819f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/58ba282a5011/nihms736819f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/b044c377d883/nihms736819f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/51f6f84fe4b1/nihms736819f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/5dfc1cb57717/nihms736819f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/0a4da9ba9bce/nihms736819f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/f332f4591d86/nihms736819f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/58ba282a5011/nihms736819f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/b044c377d883/nihms736819f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/51f6f84fe4b1/nihms736819f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/5dfc1cb57717/nihms736819f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/0a4da9ba9bce/nihms736819f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8902/4669197/f332f4591d86/nihms736819f6.jpg

相似文献

1
The spin trapping of superoxide and hydroxyl free radicals with DMPO (5,5-dimethylpyrroline-N-oxide): more about iron.用DMPO（5,5 - 二甲基吡咯啉 - N - 氧化物）对超氧自由基和羟基自由基进行自旋捕捉：更多关于铁的研究
Free Radic Res Commun. 1993;19 Suppl 1(0 1):S79-87. doi: 10.3109/10715769309056s79.
2
Superoxide dismutase-like activities of copper(II) complexes tested in serum.在血清中测试的铜（II）配合物的超氧化物歧化酶样活性。
Biochim Biophys Acta. 1987 Sep 24;915(2):267-76. doi: 10.1016/0167-4838(87)90309-8.
3
Spin traps inhibit formation of hydrogen peroxide via the dismutation of superoxide: implications for spin trapping the hydroxyl free radical.自旋捕获剂通过超氧化物的歧化作用抑制过氧化氢的形成：对自旋捕获羟基自由基的启示。
Biochim Biophys Acta. 1991 Oct 31;1075(3):213-22. doi: 10.1016/0304-4165(91)90269-m.
4
Kinetic studies on spin trapping of superoxide and hydroxyl radicals generated in NADPH-cytochrome P-450 reductase-paraquat systems. Effect of iron chelates.NADPH-细胞色素P-450还原酶-百草枯体系中产生的超氧自由基和羟基自由基自旋捕获的动力学研究。铁螯合物的影响。
J Biol Chem. 1990 Jan 15;265(2):652-9.
5
Spin-trapping and human neutrophils. Limits of detection of hydroxyl radical.自旋捕获与人类中性粒细胞。羟基自由基的检测限
J Biol Chem. 1989 Jul 25;264(21):12299-302.
6
Hydroxyl radical generation by red tide algae.赤潮藻类产生羟基自由基。
Arch Biochem Biophys. 1992 Apr;294(1):38-43. doi: 10.1016/0003-9861(92)90133-h.
7
Spin trap studies on the decomposition of peroxynitrite.关于过氧亚硝酸盐分解的自旋捕集研究。
Arch Biochem Biophys. 1995 Aug 1;321(1):31-9. doi: 10.1006/abbi.1995.1364.
8
Characterization of the high-resolution ESR spectra of superoxide radical adducts of 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide (DEPMPO) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Analysis of conformational exchange.5-(二乙氧基磷酰基)-5-甲基-1-吡咯啉 N-氧化物（DEPMPO）和 5,5-二甲基-1-吡咯啉 N-氧化物（DMPO）超氧自由基加合物的高分辨率电子自旋共振光谱表征。构象交换分析。
Free Radic Res. 2005 Aug;39(8):825-36. doi: 10.1080/10715760500155688.
9
Degradation of DMPO adducts from hydroxyl and 1-hydroxyethyl radicals by rat liver microsomes.
Free Radic Res. 1996 Dec;25(6):467-74. doi: 10.3109/10715769609149069.
10
Quantitative aspects of ESR and spin trapping of hydroxyl radicals and hydrogen atoms in gamma-irradiated aqueous solutions.γ辐照水溶液中羟基自由基和氢原子的电子自旋共振定量分析及自旋捕获
Radiat Res. 1984 Nov;100(2):222-34.

引用本文的文献

1
Photocatalytic Partial Water Oxidation Promoted by a Hydrogen Acceptor-Hydroxyl Mediator Couple.氢受体-羟基介质对促进的光催化部分水氧化
Adv Sci (Weinh). 2025 Feb;12(6):e2410680. doi: 10.1002/advs.202410680. Epub 2024 Dec 20.
2
Real-time investigation of reactive oxygen species and radicals evolved from operating Fe-N-C electrocatalysts during the ORR: potential dependence, impact on degradation, and structural comparisons.氧还原反应过程中运行的铁-氮-碳电催化剂产生的活性氧物种和自由基的实时研究：电位依赖性、对降解的影响及结构比较
Chem Sci. 2024 May 31;15(26):10036-10045. doi: 10.1039/d4sc01553c. eCollection 2024 Jul 3.
3
Electrocatalytic ORR-coupled ammoximation for efficient oxime synthesis.用于高效肟合成的电催化氧还原耦合氨肟化反应。
Sci Adv. 2024 May 24;10(21):eado1755. doi: 10.1126/sciadv.ado1755.
4
detection of reactive oxygen species spontaneously generated on lead acid battery anodes: a pathway for degradation and self-discharge at open circuit.铅酸电池阳极上自发产生的活性氧物种的检测：开路时降解和自放电的一条途径
Chem Sci. 2023 Oct 18;14(43):12292-12298. doi: 10.1039/d3sc04736a. eCollection 2023 Nov 8.
5
Resolving the Mechanism for HO Decomposition over Zr(IV)-Substituted Lindqvist Tungstate: Evidence of Singlet Oxygen Intermediacy.解析Zr(IV)取代的Lindqvist钨酸盐上HO分解的机理：单线态氧中间体的证据。
ACS Catal. 2023 Jul 24;13(15):10324-10339. doi: 10.1021/acscatal.3c02416. eCollection 2023 Aug 4.
6
Electro-assisted methane oxidation to formic acid via in-situ cathodically generated HO under ambient conditions.在环境条件下通过原位阴极生成的羟基自由基实现电辅助甲烷氧化制甲酸。
Nat Commun. 2023 Aug 5;14(1):4704. doi: 10.1038/s41467-023-40415-6.
7
Facilitated Photocatalytic Degradation of Rhodamine B over One-Step Synthesized Honeycomb-Like BiFeO/g-CN Catalyst.一步合成蜂窝状BiFeO/g-CN催化剂光催化降解罗丹明B
Nanomaterials (Basel). 2022 Nov 10;12(22):3970. doi: 10.3390/nano12223970.
8
Real-Time Detection of Hydroxyl Radical Generated at Operating Electrodes via Redox-Active Adduct Formation Using Scanning Electrochemical Microscopy.利用扫描电化学显微镜通过氧化还原活性加合物形成实时检测工作电极上生成的羟基自由基。
J Am Chem Soc. 2022 Oct 19;144(41):18896-18907. doi: 10.1021/jacs.2c06278. Epub 2022 Oct 10.
9
Fighting Antibiotic-Resistant Bacterial Infections by Surface Biofunctionalization of 3D-Printed Porous Titanium Implants with Reduced Graphene Oxide and Silver Nanoparticles.通过减少氧化石墨烯和银纳米粒子的 3D 打印多孔钛植入物表面生物功能化来对抗抗生素耐药性细菌感染。
Int J Mol Sci. 2022 Aug 16;23(16):9204. doi: 10.3390/ijms23169204.
10
The Cytotoxicity of Doxorubicin Can Be Accelerated by a Combination of Hyperthermia and 5-Aminolevulinic Acid.高温与5-氨基乙酰丙酸联合应用可加速阿霉素的细胞毒性。
Antioxidants (Basel). 2021 Sep 27;10(10):1531. doi: 10.3390/antiox10101531.

本文引用的文献

1
The kinetics of the reaction of superoxide radical with Fe(III) complexes of EDTA, DETAPAC and HEDTA.超氧自由基与 EDTA、DETAPAC 和 HEDTA 的 Fe(III)配合物反应的动力学。
FEBS Lett. 1983 Jul 11;158(1):143-6. doi: 10.1016/0014-5793(83)80695-4.
2
Ascorbate free radical as a marker of oxidative stress: an EPR study.抗坏血酸自由基作为氧化应激的标志物：一项电子顺磁共振研究。
Free Radic Biol Med. 1993 Jan;14(1):49-55. doi: 10.1016/0891-5849(93)90508-r.
3
Production of hydroxyl radical by decomposition of superoxide spin-trapped adducts.超氧化物自旋捕获加合物分解产生羟基自由基。
Mol Pharmacol. 1982 Mar;21(2):262-5.
4
Quantitative aspects of ESR and spin trapping of hydroxyl radicals and hydrogen atoms in gamma-irradiated aqueous solutions.γ辐照水溶液中羟基自由基和氢原子的电子自旋共振定量分析及自旋捕获
Radiat Res. 1984 Nov;100(2):222-34.
5
Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).超氧化物歧化酶。红细胞铜蛋白（血铜蛋白）的酶功能。
J Biol Chem. 1969 Nov 25;244(22):6049-55.
6
In the absence of catalytic metals ascorbate does not autoxidize at pH 7: ascorbate as a test for catalytic metals.在没有催化金属存在的情况下，抗坏血酸盐在pH值为7时不会自动氧化：抗坏血酸盐可作为催化金属的一种检测方法。
J Biochem Biophys Methods. 1988 May;16(1):27-40. doi: 10.1016/0165-022x(88)90100-5.
7
Superoxide reaction with nitroxide spin-adducts.超氧化物与氮氧化物自旋加合物的反应。
Free Radic Biol Med. 1989;6(2):141-8. doi: 10.1016/0891-5849(89)90111-1.
8
Hydroxyl radical is not a product of the reaction of xanthine oxidase and xanthine. The confounding problem of adventitious iron bound to xanthine oxidase.
J Biol Chem. 1990 Oct 15;265(29):17533-8.
9
Ascorbate oxidation: UV absorbance of ascorbate and ESR spectroscopy of the ascorbyl radical as assays for iron.抗坏血酸氧化：以抗坏血酸的紫外吸光度和抗坏血酸自由基的电子顺磁共振波谱法作为铁的测定方法。
Free Radic Res Commun. 1990;10(1-2):5-9. doi: 10.3109/10715769009145927.
10
On the reaction of superoxide with DMPO/.OOH.
Free Radic Res Commun. 1990;10(1-2):11-5. doi: 10.3109/10715769009145928.