用DMPO（5,5 - 二甲基吡咯啉 - N - 氧化物）对超氧自由基和羟基自由基进行自旋捕捉：更多关于铁的研究
The spin trapping of superoxide and hydroxyl free radicals with DMPO (5,5-dimethylpyrroline-N-oxide): more about iron.
作者信息
Buettner G R
机构信息
ESR Facility/EMRB 68, College of Medicine, University of Iowa, Iowa City 52242.
出版信息
Free Radic Res Commun. 1993;19 Suppl 1(0 1):S79-87. doi: 10.3109/10715769309056s79.
Abstract
The reaction of superoxide with the spin trap DMPO (5,5-dimethylpyrroline-N-oxide) is widely used to study superoxide production as well as issues associated with the superoxide-related formation of hydroxyl radical. However, the interpretation of observed intensities of DMPO/.OOH and DMPO/.OH signals in electron paramagnetic resonance spin trapping experiments is not without its difficulties. In this paper, I report experiments that demonstrate: 1. That the flux of superoxide formation in a DMPO spin trapping experiment can alter the apparent importance of weak DMPO/.OH signals; 2. That iron can influence the DMPO/.OOH spin trapping results; 3. That there is very little spontaneous breakdown of DMPO/.OOH to form DMPO/.OH.
摘要
超氧化物与自旋捕捉剂DMPO（5,5 - 二甲基吡咯啉 - N - 氧化物）的反应被广泛用于研究超氧化物的产生以及与超氧化物相关的羟基自由基形成的相关问题。然而，在电子顺磁共振自旋捕捉实验中，对观察到的DMPO/.OOH和DMPO/.OH信号强度的解释并非没有困难。在本文中，我报告的实验表明：1. 在DMPO自旋捕捉实验中超氧化物形成的通量可以改变微弱的DMPO/.OH信号的表观重要性；2. 铁可以影响DMPO/.OOH自旋捕捉结果；3. DMPO/.OOH自发分解形成DMPO/.OH的情况非常少。
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