Shoyama Y, Fukada T, Tanaka T, Kusai A, Nojima K
Faculty of Pharmaceutical Science, Kyushu University, Fukuoka, Japan.
Biol Pharm Bull. 1993 Oct;16(10):1051-3. doi: 10.1248/bpb.16.1051.
Opium alkaloids (thebaine, codeine and morphine) have been conjugated with bovine serum albumin (BSA) to give individual antigen conjugates which are analyzed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It became clear that 9 molecules of thebaine were contained in a thebaine-BSA conjugate. Codeine and morphine contents in individual conjugates were determined to be 12 and 6 molecules, respectively.
鸦片生物碱（蒂巴因、可待因和吗啡）已与牛血清白蛋白（BSA）结合，形成单个抗原结合物，并用基质辅助激光解吸/电离（MALDI）质谱法进行分析。结果表明，蒂巴因-BSA结合物中含有9个蒂巴因分子。单个结合物中的可待因和吗啡含量分别确定为12个分子和6个分子。
