制备抗人参主要成分人参皂苷 Rg1 的单克隆抗体及其特性。一种人参皂苷的单克隆抗体。
Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin.
机构信息
Department of Pharmacognosy, Graduate school of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higahsi-ku, Fukuoka, 812,-8582, Japan.
出版信息
Cytotechnology. 2000 Nov;34(3):197-204. doi: 10.1023/A:1008162703957.
Abstract
The ratio of hapten and bovine serum albumin in an antigenconjugate was determined by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Ahybridoma secreting monoclonal antibody against ginsenosideRg(1) was produced by fusing sprenocytes immunized with aginsenoside Rg(1)-bovine serum albumin conjugate withHAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very smallcross-reaction appeared with ginsenoside Re. The full measuringrange of the assay extends from 0.3 mug ml(-1) to 10 mug ml(-1) ofginsenoside Rg(1).
摘要
抗原缀合物中半抗原与牛血清白蛋白的比例通过基质辅助激光解吸/电离飞行时间质谱法确定。用人参皂苷 Rg(1)-牛血清白蛋白缀合物免疫的脾细胞与 HAT 敏感的小鼠骨髓瘤细胞系 P3-X63-Ag8-653 融合，产生了一株分泌针对人参皂苷 Rg(1)的单克隆抗体的杂交瘤。该方法与人参皂苷 Re 有很小的交叉反应。测定的全测量范围从 0.3 微克/毫升到 10 微克/毫升的人参皂苷 Rg(1)。
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本文引用的文献
1
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Plant Cell Rep. 1997 Apr;16(7):450-453. doi: 10.1007/BF01092764.
2
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3
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4
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7
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Chem Pharm Bull (Tokyo). 1998 Jul;46(7):1144-7. doi: 10.1248/cpb.46.1144.
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