生产单克隆抗体并开发一种用于检测索拉玛灵的 ELISA 方法。
Production of monoclonal antibodies and development of an ELISA for solamargine.
机构信息
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812, Fukuoka, Japan.
出版信息
Cytotechnology. 1995 Jan;18(3):153-8. doi: 10.1007/BF00767762.
The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibodies against solamargine was produced by fusing splenocytes immunized with a solamargine-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. Extensive cross-reaction of anti-solamargine antibodies against solasonine appeared. Aglycone of solamargine, solasodine cross-reacted with anti-solamargine antibodies resulting in a 43.8% cross-reaction. Insignificant cross-reaction appeared with tomatine (2.06%). The full measuring range of the assay extends from 57.5 pmol ml(-1) to 11.5 nmol ml(-1) of solamargine.
用基质辅助激光解吸/电离质谱法测定抗原缀合物中半抗原和牛血清白蛋白的比例。用 solamargine-牛血清白蛋白缀合物免疫的脾细胞与 HAT 敏感的小鼠骨髓瘤细胞系 P3-X63-Ag8-653融合，产生了分泌抗 solamargine 单克隆抗体的杂交瘤。抗 solamargine 抗体对 solasonine 表现出广泛的交叉反应。 solamargine 的苷元，甾体皂甙元与抗 solamargine 抗体交叉反应，导致 43.8%的交叉反应。与番茄碱（2.06%）的交叉反应不明显。该测定方法的全测量范围从 57.5 pmol ml(-1)到 11.5 nmol ml(-1)的 solamargine。
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