制备西红花苷单克隆抗体及其鉴定。
Preparation of monoclonal antibody against crocin and its characterization.
机构信息
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka, 812, Japan.
出版信息
Cytotechnology. 1999 Jan;29(1):65-70. doi: 10.1023/A:1007993615489.
Abstract
Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG2a and IgG2b possessing lambda light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.
摘要
合成了 3 种西红花苷载体蛋白缀合物，并通过基质辅助激光解吸/电离飞行时间质谱法测定了它们的半抗原数。用西红花半琥珀酸-牛血清白蛋白缀合物免疫脾细胞与 HAT 敏感的小鼠骨髓瘤细胞系 P3-X63-Ag8-653 融合，制备了 3 株针对西红花苷的单克隆抗体。它们分别被鉴定为 IgG2a 和 IgG2b，均具有 lambda 轻链。讨论了它们对西红花酸糖苷的广泛反应性。
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本文引用的文献
1
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Cytotechnology. 1995 Jan;19(1):55-61. doi: 10.1007/BF00749755.
2
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Cytotechnology. 1995 Jan;18(3):153-8. doi: 10.1007/BF00767762.
3
A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine.使用抗茄碱单克隆抗体通过薄层色谱免疫染色法简单测定甾体生物碱苷。
FEBS Lett. 1997 Mar 10;404(2-3):279-82. doi: 10.1016/s0014-5793(97)00106-3.
4
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J Immunoassay. 1996 Nov;17(4):321-42. doi: 10.1080/01971529608005796.
5
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Cancer Lett. 1996 Feb 27;100(1-2):23-30. doi: 10.1016/0304-3835(95)04067-6.
6
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