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本文引用的文献

1
Production of monoclonal antibodies and ELISA for thebaine and codeine.用于蒂巴因和可待因的单克隆抗体和 ELISA 的生产。
Cytotechnology. 1995 Jan;19(1):55-61. doi: 10.1007/BF00749755.
2
Production of monoclonal antibodies and development of an ELISA for solamargine.生产单克隆抗体并开发一种用于检测索拉玛灵的 ELISA 方法。
Cytotechnology. 1995 Jan;18(3):153-8. doi: 10.1007/BF00767762.
3
A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine.使用抗茄碱单克隆抗体通过薄层色谱免疫染色法简单测定甾体生物碱苷。
FEBS Lett. 1997 Mar 10;404(2-3):279-82. doi: 10.1016/s0014-5793(97)00106-3.
4
Monoclonal antibody based enzyme immunoassay for marihuana (cannabinoid) compounds.基于单克隆抗体的大麻（大麻素）化合物酶免疫测定法。
J Immunoassay. 1996 Nov;17(4):321-42. doi: 10.1080/01971529608005796.
5
Crocin, safranal and picrocrocin from saffron (Crocus sativus L.) inhibit the growth of human cancer cells in vitro.藏红花（番红花）中的藏红花素、藏红花醛和苦藏花素在体外可抑制人类癌细胞的生长。
Cancer Lett. 1996 Feb 27;100(1-2):23-30. doi: 10.1016/0304-3835(95)04067-6.
6
Inhibitory effect of crocetin on intracellular nucleic acid and protein synthesis in malignant cells.
Toxicol Lett. 1994 Feb 1;70(2):243-51. doi: 10.1016/0378-4274(94)90168-6.
7
Direct determination of opium alkaloid-bovine serum albumin conjugate by matrix-assisted laser desorption/ionization mass spectrometry.通过基质辅助激光解吸/电离质谱法直接测定鸦片生物碱-牛血清白蛋白缀合物
Biol Pharm Bull. 1993 Oct;16(10):1051-3. doi: 10.1248/bpb.16.1051.
8
Effects of Crocus sativus L. on the ethanol-induced impairment of passive avoidance performances in mice.藏红花对乙醇诱导的小鼠被动回避行为损伤的影响。
Biol Pharm Bull. 1994 Feb;17(2):217-21. doi: 10.1248/bpb.17.217.
9
Post-harvest degradation of carotenoid glucose esters in saffron.
Planta Med. 1994 Oct;60(5):438-40. doi: 10.1055/s-2006-959527.
10
Crocin (crocetin di-gentiobiose ester) prevents the inhibitory effect of ethanol on long-term potentiation in the dentate gyrus in vivo.藏红花素（西红花酸二龙胆二糖酯）可防止乙醇对体内齿状回长时程增强的抑制作用。
J Pharmacol Exp Ther. 1994 Nov;271(2):703-7.

制备西红花苷单克隆抗体及其鉴定。

Preparation of monoclonal antibody against crocin and its characterization.

机构信息

Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka, 812, Japan.

出版信息

Cytotechnology. 1999 Jan;29(1):65-70. doi: 10.1023/A:1007993615489.

DOI:10.1023/A:1007993615489
PMID:19003338
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3449468/
Abstract

Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG2a and IgG2b possessing lambda light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.

摘要

合成了 3 种西红花苷载体蛋白缀合物，并通过基质辅助激光解吸/电离飞行时间质谱法测定了它们的半抗原数。用西红花半琥珀酸-牛血清白蛋白缀合物免疫脾细胞与 HAT 敏感的小鼠骨髓瘤细胞系 P3-X63-Ag8-653 融合，制备了 3 株针对西红花苷的单克隆抗体。它们分别被鉴定为 IgG2a 和 IgG2b，均具有 lambda 轻链。讨论了它们对西红花酸糖苷的广泛反应性。