Sundaram S G, Milner J A
Department of Nutrition, Pennsylvania State University, University Park 16802.
Cancer Lett. 1993 Oct 15;74(1-2):85-90. doi: 10.1016/0304-3835(93)90048-e.
Six organosulfur compounds found in garlic were examined for their ability to alter the growth of canine mammary tumor cells (CMT-13) in culture. Water-soluble organosulfur compounds (S-allyl-cysteine, S-ethyl-cysteine and S-propyl-cysteine) did not significantly alter the growth of CMT-13 cells when added to cultures at 1.0 mM or less. However, oil-soluble organosulfur compounds (diallyl sulfide, diallyl disulfide and diallyl trisulfide) markedly inhibited growth. Increasing addition of diallyl disulfide (DADS) resulted in a progressive decrease in CMT-13 cell growth. Addition of glutathione before DADS markedly decreased the severity of the growth inhibition. Treatment with DL-buthionine-SR-sulfoxamine, a specific inhibitor of glutathione synthesis, accentuated the growth inhibition caused by DADS. These studies show that some organosulfur compounds found in garlic are effective inhibitors of the growth of the neoplastic CMT-13 cell. The inhibitory effects of these compounds are modified by intracellular glutathione.
研究了大蒜中发现的六种有机硫化合物改变培养的犬乳腺肿瘤细胞（CMT-13）生长的能力。当以1.0 mM或更低浓度添加到培养物中时，水溶性有机硫化合物（S-烯丙基半胱氨酸、S-乙基半胱氨酸和S-丙基半胱氨酸）不会显著改变CMT-13细胞的生长。然而，油溶性有机硫化合物（二烯丙基硫醚、二烯丙基二硫醚和二烯丙基三硫醚）显著抑制生长。增加二烯丙基二硫醚（DADS）的添加量会导致CMT-13细胞生长逐渐减少。在DADS之前添加谷胱甘肽可显著降低生长抑制的严重程度。用谷胱甘肽合成的特异性抑制剂DL-丁硫氨酸-SR-亚砜胺处理可加剧DADS引起的生长抑制。这些研究表明，大蒜中发现的一些有机硫化合物是肿瘤性CMT-13细胞生长的有效抑制剂。这些化合物的抑制作用会被细胞内谷胱甘肽修饰。
