O'Dowd B F, Heiber M, Chan A, Heng H H, Tsui L C, Kennedy J L, Shi X, Petronis A, George S R, Nguyen T
Addiction Research Foundation, Toronto, Ontario, Canada.
Gene. 1993 Dec 22;136(1-2):355-60. doi: 10.1016/0378-1119(93)90495-o.
We report the cloning of a gene, intronless in its coding region, which we have named APJ. This gene was cloned using the polymerase chain reaction (PCR), with a set of primers designed on the basis of the conservation that members of G protein-coupled receptors (GPCR) have in their transmembrane (TM) regions. The putative receptor protein, APJ, shares closest identity to the angiotensin receptor (AT1) ranging from 40 to 50% in the hydrophobic TM regions of these receptors. The transcripts for this gene were detected in many regions of the brain. PCR analysis of somatic cell lines found APJ-related sequences to be only present on chromosome 11, and high-resolution mapping by fluorescence in situ hybridization (FISH) sublocalized APJ on band q12.
我们报道了一个编码区无内含子的基因的克隆，我们将其命名为APJ。该基因是利用聚合酶链反应（PCR）克隆的，所用的一组引物是根据G蛋白偶联受体（GPCR）成员在其跨膜（TM）区域的保守性设计的。推测的受体蛋白APJ与血管紧张素受体（AT1）的同源性最高，在这些受体的疏水TM区域中为40%至50%。在大脑的许多区域都检测到了该基因的转录本。对体细胞系的PCR分析发现，APJ相关序列仅存在于11号染色体上，通过荧光原位杂交（FISH）进行的高分辨率定位将APJ定位于q12带。
