人体呼出一氧化氮的测量。
Measurement of exhaled nitric oxide in man.
作者信息
Borland C, Cox Y, Higenbottam T
机构信息
Department of Respiratory Physiology, Papworth Hospital, Cambridge.
出版信息
Thorax. 1993 Nov;48(11):1160-2. doi: 10.1136/thx.48.11.1160.
BACKGROUND
Nitric oxide is released from pulmonary endothelial cells and contributes to the low pulmonary vascular resistance. The resistance pulmonary arteries are in close anatomical proximity to membranous airways, so it is likely that some pulmonary endothelial nitric oxide will enter the airspace to allow its measurement in the exhaled breath.
METHODS
Exhaled air was collected from a single full exhalation and during tidal breathing. This was analysed for concentrations of nitric oxide, nitrogen dioxide, and carbon dioxide to give alveolar (FA) and mixed expired (FE) concentrations. Eight normal subjects were studied and laboratory air was similarly analysed using, respectively, chemiluminescent and infrared analysers.
RESULTS
There was no relation between FA concentrations and the laboratory air concentrations. From the single breath, the ratio of (Fano/Faco2) x (Feco2/Feno) had a mean value of 0.92 (95% confidence interval 0.7 to 1.14). As this does not differ from unity, nitric oxide is likely to be derived from the same regions of the lungs as carbon dioxide. During tidal breathing the Feno ranged from 8.3 to 20.3 parts per billion.
CONCLUSIONS
It is possible to measure endogenous pulmonary nitric oxide production in the exhaled air in man.
背景
一氧化氮由肺内皮细胞释放，有助于降低肺血管阻力。肺阻力动脉在解剖位置上与膜性气道紧密相邻，因此一些肺内皮一氧化氮很可能进入气腔，以便在呼出气体中进行测量。
方法
从一次完整呼气和潮式呼吸过程中收集呼出气体。分析其中一氧化氮、二氧化氮和二氧化碳的浓度，得出肺泡（FA）和混合呼出气体（FE）浓度。对8名正常受试者进行了研究，并分别使用化学发光分析仪和红外分析仪对实验室空气进行了类似分析。
结果
FA浓度与实验室空气浓度之间无相关性。单次呼吸时，（Fano/Faco2）×（Feco2/Feno）的比值平均值为0.92（95%置信区间为0.7至1.14）。由于该值与1无差异，一氧化氮可能与二氧化碳来自肺的相同区域。潮式呼吸过程中，呼出气体中一氧化氮浓度范围为8.3至20.3 ppb。
结论
有可能在人体呼出气体中测量内源性肺一氧化氮的产生。
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