Nye L, Yeo T H, Chan V, Goldie D, Landon J
J Clin Pathol. 1975 Nov;28(11):915-9. doi: 10.1136/jcp.28.11.915.
The stability of thyroxine and triiodothyronine in serum has been investigated. Apparent levels of total thryroxine, as determined by two different protein-binding assays employing thyroxine-binding globulin as the binding protein, increased significantly in serum and plasma samples stored at room temperature and were signficantly lower in haemolysed samples. Values did not change significantly in samples stored at 4 degrees C, nor in samples stored at room temperature when determined by radioimmunoassay. Total triiodothyronine levels, as determined by radioimmunoassay, fell slightly on storage. Failure to appreciate the effect of storing samples at room temperature on apparent levels of total thyroxine, as determined by some protein-binding assays, could lead to an incorrect assessment of thyroid status.
已对血清中甲状腺素和三碘甲状腺原氨酸的稳定性进行了研究。采用甲状腺素结合球蛋白作为结合蛋白的两种不同蛋白质结合分析法测定的总甲状腺素表观水平，在室温下储存的血清和血浆样本中显著升高，而在溶血样本中则显著降低。通过放射免疫分析法测定时，4℃储存的样本以及室温储存的样本中的数值均无显著变化。通过放射免疫分析法测定的总三碘甲状腺原氨酸水平在储存时略有下降。若未认识到室温储存样本对某些蛋白质结合分析法测定的总甲状腺素表观水平的影响，可能会导致对甲状腺状态的错误评估。
