使用鸟嘌呤 - 胞嘧啶（GC）特异性荧光染料放线菌素A3作为精子形态不良的指标。
Use of the guanine-cytosine (GC) specific fluorochrome, chromomycin A3, as an indicator of poor sperm morphology.
作者信息
Bianchi P G, Manicardi G, Bizzaro D, Campana A, Bianchi U, Sakkas D
机构信息
Clinic of Infertility and Gynaecological Endocrinology-WHO Collaborating Centre, University Hospital of Geneva, Switzerland.
出版信息
J Assist Reprod Genet. 1996 Mar;13(3):246-50. doi: 10.1007/BF02065944.
PURPOSE
We have previously postulated that the chromomycin A3 (CMA3) fluorochrome allows an indirect visualization of sperm chromatin packaging quality and partially denatured sperm DNA. In this study we investigate the relationship between CMA3 positivity and sperm morphology. We also present data on the association between sperm morphology and the presence of endogenous nicks in sperm DNA.
METHODS
Semen samples were examined from 81 males of the couples who were consulting for infertility treatment. CMA3 fluorescence was assessed for all samples, while in 24 sperm samples we also examined for the presence of endogenous nicks in the sperm DNA.
RESULTS
When sperm morphology was less than 20% normal in a patient, the level of CMA3 fluorescence and presence of endogenous nicks were significantly higher than in patients with a higher incidence of morphologically normal sperm.
CONCLUSIONS
CMA3 could be used as an adjunct to the assessment of morphology as an evaluation method for poor sperm. Its value in predicting fertilizing ability when using either SUZI or ICSI awaits to be answered.
目的
我们之前曾推测，放线菌素A3（CMA3）荧光染料可间接显示精子染色质包装质量以及部分变性的精子DNA。在本研究中，我们调查了CMA3阳性与精子形态之间的关系。我们还提供了关于精子形态与精子DNA中内源性切口存在之间关联的数据。
方法
对81名因不育症前来咨询治疗的男性夫妇的精液样本进行检查。对所有样本评估CMA3荧光，而在24份精子样本中，我们还检查了精子DNA中内源性切口的存在情况。
结果
当患者精子形态正常率低于20%时，CMA3荧光水平和内源性切口的存在情况显著高于形态正常精子发生率较高的患者。
结论
CMA3可作为评估精子质量差的形态学评估方法的辅助手段。其在使用SUZI或ICSI时预测受精能力的价值有待进一步研究。
Zotero 插件