Bosch F H, Werre J M, Schipper L, Roerdinkholder-Stoelwinder B, Huls T, Willekens F L, Wichers G, Halie M R
Department of Internal Medicine, Rijnstate Hospital, Arnhem, The Netherlands.
Eur J Haematol. 1994 Jan;52(1):35-41. doi: 10.1111/j.1600-0609.1994.tb01282.x.
Red blood cell (RBC) deformability was determined with an ektacytometer in fractions separated on the basis of differences in cell volume or density. Deformability was measured with ektacytometry (rpm-scan and osmo-scan). We studied three groups of RBC fractions:1. By counterflow centrifugation we obtained fractions of different cell age which showed a slight decrease in mean corpuscular haemoglobin concentration (MCHC) and an increase in surface-to-volume (S/V) ratio in fractions with older cells. 2. By Percoll fractionation fractions were obtained which showed a pronounced increase in (MCHC) but no change in S/V ratio. 3. By a combination of both fractionation techniques, fractions were obtained which showed an increased MCHC and an increase in S/V ratio. Deformability in group 1,2 and 3 showed respectively no change, a moderate decrease and a pronounced decrease in fractions of older cells. A decline in deformability occurs during the aging process of the red blood cell. This decline in deformability in old red cells is greater than originally thought. This decline is the result of an increase in haemoglobin concentration and a second factor, probably a decrease in membrane elasticity.
使用血细胞变形性测定仪，在根据细胞体积或密度差异分离出的组分中测定红细胞（RBC）的变形性。通过血细胞变形性测定法（转速扫描和渗透压扫描）测量变形性。我们研究了三组红细胞组分：1. 通过逆流离心，我们获得了不同细胞年龄的组分，这些组分显示平均红细胞血红蛋白浓度（MCHC）略有下降，且在细胞年龄较大的组分中表面积与体积（S/V）比增加。2. 通过Percoll分级分离获得的组分显示（MCHC）显著增加，但S/V比无变化。3. 通过两种分级分离技术的组合，获得的组分显示MCHC增加且S/V比增加。第1、2和3组中，细胞年龄较大的组分的变形性分别无变化、适度下降和显著下降。在红细胞衰老过程中会出现变形性下降。衰老红细胞中这种变形性下降比原先认为的更为严重。这种下降是血红蛋白浓度增加以及第二个因素（可能是膜弹性降低）的结果。
