Rose B I, Scott B
Fertility and Reproductive Health Services, Allentown, Pennsylvania.
Fertil Steril. 1994 Feb;61(2):341-8. doi: 10.1016/s0015-0282(16)56529-7.
To evaluate the effect on the function on human sperm of overnight incubation with mycoplasma species.
Prospective controlled in vitro study.
Community-based sperm bank and andrology laboratory.
Percoll-separated sperm were incubated overnight with a low densities of Myco-plasma hominis and Ureaplasma urealyticum serotypes.
Motility, midpiece and tail morphology, hyperactivation after overnight capacitation, and acrosome status after incubation with calcium ionophore.
Overnight incubation with mycoplasma species resulted in small but highly statistically significant differences in motility, morphology, hyperactivation after overnight capacitation, and proportion acrosome reacted after incubation with calcium ionophore compared with controls.
Incubation with mycoplasma species impairs sperm physiology. Identification and treatment of mycoplasma may augment a physiology-based infertility evaluation.
评估支原体与人类精子过夜孵育对精子功能的影响。
前瞻性对照体外研究。
社区精子库及男科实验室。
将经Percoll分离的精子与低密度的人型支原体和解脲脲原体血清型过夜孵育。
活力、中段及尾部形态、过夜获能后的超激活状态以及与钙离子载体孵育后的顶体状态。
与对照组相比，支原体过夜孵育导致精子在活力、形态、过夜获能后的超激活状态以及与钙离子载体孵育后的顶体反应比例方面出现虽小但具有高度统计学意义的差异。
支原体孵育会损害精子生理功能。支原体的鉴定与治疗可能会加强基于生理的不孕症评估。
