Shadix L C, Dunnigan M E, Rice E W
Technical Support Division, United States Environmental Protection Agency, Cincinnati, Ohio 45268.
Can J Microbiol. 1993 Nov;39(11):1066-70. doi: 10.1139/m93-161.
A two-step membrane filter procedure was evaluated to determine the ability to differentiate Escherichia coli from other coliform bacteria recovered from water. M-Endo LES agar incubated at 35 degrees C for 24 +/- 2 h was used as the initial isolation medium. Membranes containing coliform colonies were transferred to nutrient agar plus 4-methylumbelliferyl beta-D-glucuronide (MUG) and incubated for an additional 4 h at 35 degrees C. Escherichia coli colonies were distinguished by fluorescence when viewed under a long-wavelength ultraviolet light. A total of 119 MUG-positive colonies were isolated from 15 water sources, of which 115 (96.6%) were identified as E. coli. An examination of 182 pure culture environmental E. coli isolates revealed that 167 isolates (91.8%) exhibited fluorescence on the nutrient agar plus MUG medium. Survivors of E. coli cultures exposed to chlorination were also capable of producing a positive MUG reaction.
对一种两步膜过滤程序进行了评估，以确定其区分从水中回收的大肠杆菌与其他大肠菌群细菌的能力。将在35℃下培养24±2小时的M-远藤LES琼脂用作初始分离培养基。含有大肠菌群菌落的膜转移至营养琼脂加4-甲基伞形酮基β-D-葡萄糖醛酸苷（MUG）中，并在35℃下再培养4小时。在长波紫外线下观察时，大肠杆菌菌落可通过荧光进行区分。从15个水源中共分离出119个MUG阳性菌落，其中115个（96.6%）被鉴定为大肠杆菌。对182株环境大肠杆菌纯培养物的检查显示，167株（91.8%）在营养琼脂加MUG培养基上呈现荧光。暴露于氯化作用的大肠杆菌培养物的存活菌也能够产生阳性MUG反应。
