孤立骨髓瘤细胞核中的RNA转运。从膜剥脱细胞核的转运。
RNA transport in isolated myeloma nuclei. Transport from membrane-denuded nuclei.
作者信息
Stuart S E, Clawson G A, Rottman F M, Patterson R J
出版信息
J Cell Biol. 1977 Jan;72(1):57-66. doi: 10.1083/jcb.72.1.57.
Abstract
Nuclei prepared from MOPC-21 cells were treated with the nonionic detergents Triton X-100 or Nonidet P-40. Chemical analysis revealed that nearly 90% of the nuclear phospholipid was removed by detergent treatment. The membrane-denuded nuclei remained intact with preservation of nuclear pore complexes as demonstrated by electron microscopy. Ribonucleic acid transport from detergent-treated nuclei proceeded at the same rate and to the same extent as in control nuclei. Normal nuclear restriction of nucleic acids was unaltered by removal of the nuclear membranes. The effect of temperature on transport of RNA from freshly isolated myeloma nuclei with intact nuclear envelopes was studied. No temperature transition was associated with the transport process. These data indicate that the transport of macromolecules from isolated myeloma nuclei is independent of the nuclear membrane.
摘要
从MOPC - 21细胞制备的细胞核用非离子去污剂Triton X - 100或Nonidet P - 40处理。化学分析表明，去污剂处理可去除近90%的核磷脂。如电子显微镜所示，去除膜后的细胞核保持完整，核孔复合体得以保留。经去污剂处理的细胞核的核糖核酸转运速率和程度与对照细胞核相同。去除核膜后，核酸的正常核限制未改变。研究了温度对具有完整核膜的新鲜分离骨髓瘤细胞核中RNA转运的影响。转运过程未伴随温度转变。这些数据表明，从分离的骨髓瘤细胞核中转运大分子与核膜无关。
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