Sekhon A S, Garg A K, Padhye A A, Hamir Z
National Centre for Human Mycotic Diseases, University of Alberta, Edmonton, Canada.
Eur J Epidemiol. 1993 Sep;9(5):553-8. doi: 10.1007/BF00209535.
The mycelial (25 degrees C) and yeast-like (37 degrees C) forms of Penicillium marneffei clinical and type strains were investigated for their in vitro susceptibility to amphotericin B (AmB), 5-fluorocytosine (5-FC), fluconazole (FLU) and itraconazole (ITZ), using Bacto antibiotic medium 3, yeast-nitrogen, Sabouraud's dextrose (pH 5.7) and high resolution (pH 7.1) broth media (1ml/tube), respectively. Results indicated that the minimal inhibitory and minimal fungicidal concentrations (MICs and MFCs) for the mycelial cultures of P. marneffei to AmB were in the range 0.78-1.56 and 0.78-3.125 micrograms/ml, respectively, as against 3.125-25 micrograms (MICs) for the yeast form cultures. The MFCs to AmB for the yeast form were one dilution higher. The MICs to FLU were generally lower for the yeast form (6.25-25 micrograms) than the mycelial form (25-50 micrograms/ml), whereas MFCs for the mycelial cultures were > 100 micrograms as compared to 6.25-100 micrograms for their yeast form. The MICs for the mycelial form to 5-FC ranged from < 0.195-0.39 microgram. Higher MICs (6.25 micrograms) were recorded for their yeast form. The MFCs to 5-FC for the yeast form were 25-100 micrograms/ml. The MICs for the mycelial form to ITZ ranged from < 0.195 to 3.125 micrograms/ml. Higher values (< 0.195-50 micrograms) were recorded for their yeast-like form. The MFCs to ITZ for mycelial and yeast forms ranged from < 0.195-0.39 and 25-100 micrograms/ml, respectively. Results indicate that P. marneffei's yeast form is more sensitive to FLU and ITZ (8 of 10 strains) while the mycelial form displayed greater susceptibility to AmB and 5-FC. The MICs for ITZ remained steady in SD medium, pH 5.7 to 7.1. However, some strains gave higher MIC values (0.39-1.56 micrograms/ml) when tested in the HR.
研究了马尔尼菲青霉临床菌株和标准菌株在25℃时的丝状形式以及在37℃时的酵母样形式对两性霉素B（AmB）、5-氟胞嘧啶（5-FC）、氟康唑（FLU）和伊曲康唑（ITZ）的体外敏感性，分别使用细菌抗生素培养基3、酵母氮源、沙氏葡萄糖（pH 5.7）和高分辨率（pH 7.1）肉汤培养基（1ml/试管）。结果表明，马尔尼菲青霉丝状培养物对AmB的最低抑菌浓度和最低杀菌浓度（MIC和MFC）分别在0.78 - 1.56和0.78 - 3.125微克/毫升范围内，而酵母形式培养物的MIC为3.125 - 25微克。酵母形式对AmB的MFC高一个稀释度。酵母形式对FLU的MIC通常低于丝状形式（6.25 - 25微克），丝状培养物的MFC大于100微克，而酵母形式为6.25 - 100微克。丝状形式对5-FC的MIC范围为<0.195 - 0.39微克。酵母形式的MIC较高（6.25微克）。酵母形式对5-FC的MFC为25 - 100微克/毫升。丝状形式对ITZ的MIC范围为<0.195至3.125微克/毫升。酵母样形式的数值较高（<0.195 - 50微克）。丝状和酵母形式对ITZ的MFC分别为<0.195 - 0.39和25 - 100微克/毫升。结果表明，马尔尼菲青霉的酵母形式对FLU和ITZ更敏感（10株中有8株），而丝状形式对AmB和5-FC更敏感。在pH 5.7至7.1的SD培养基中，ITZ的MIC保持稳定。然而，一些菌株在高分辨率培养基中测试时给出了更高的MIC值（0.39 - 1.56微克/毫升）。
