Perparim K, Nagai H, Hashimoto A, Goto Y, Tashiro T, Nasu M
Second Department of Internal Medicine, Oita Medical University, Japan.
J Chemother. 1996 Dec;8(6):445-8. doi: 10.1179/joc.1996.8.6.445.
The minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of amphotericin B, flucytosine, miconazole, fluconazole and itraconazole against 21 isolates of Trichosporon beigelii in RPMI-1640 medium were determined using National Committee for Clinical Laboratory Standards (NCCLS) methodology in microdilution method. Most isolates were sensitive to miconazole (MIC90 0.78 microgram/ml), fluconazole (MIC90 6.25 micrograms/ml), and itraconazole (MIC90 0.19 microgram/ml), with the former being the most active agent tested (MFC90 3.12 mu/ml). Although amphotericin B inhibited most strains (MIC range, 0.78-3.12 micrograms/ml), poor fungicidal activity was observed (MFC range, 1.56-12.5 micrograms/ml) showing a pattern of relative resistance in vitro. Flucytosine showed generally poor activity against most isolates tested. These in vitro findings confirm the resistance of T.beigelii to amphotericin B and suggest that azoles may be an alternative to the former for the treatment of disseminated trichosporonosis. However, in vivo studies would better validate these in vitro findings.
采用美国国家临床实验室标准委员会（NCCLS）微量稀释法，在RPMI - 1640培养基中测定了两性霉素B、氟胞嘧啶、咪康唑、氟康唑和伊曲康唑对21株白吉利丝孢酵母的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）。大多数分离株对咪康唑（MIC90为0.78微克/毫升）、氟康唑（MIC90为6.25微克/毫升）和伊曲康唑（MIC90为0.19微克/毫升）敏感，其中咪康唑是所测试的最具活性的药物（MFC90为3.12微克/毫升）。虽然两性霉素B抑制了大多数菌株（MIC范围为0.78 - 3.12微克/毫升），但观察到其杀菌活性较差（MFC范围为1.56 - 12.5微克/毫升），显示出体外相对耐药模式。氟胞嘧啶对大多数测试分离株的活性普遍较差。这些体外研究结果证实了白吉利丝孢酵母对两性霉素B耐药，并表明唑类药物可能是治疗播散性白吉利丝孢酵母病替代两性霉素B的药物。然而，体内研究将能更好地验证这些体外研究结果。
