Ozaki T, Masuda S, Asano Y, Kondo K, Namazue J, Yamanishi K
Department of Pediatrics, Showa Hospital, Kohnan, Japan.
J Med Virol. 1994 Jan;42(1):47-51. doi: 10.1002/jmv.1890420110.
Investigation of varicella-zoster virus (VZV) DNA in 20 healthy children with varicella vaccination was carried out by using the polymerase chain reaction (PCR) and nested double PCR. Samples of peripheral blood mononuclear cells (PBMC) and throat swabs were simultaneously collected 3 times (before, 1 week, and 4 weeks after vaccination) for PCR analysis. One sample of PBMC was also obtained from each of the 12 healthy children with varicella during the acute phase as a positive control. VZV DNA was not found by the first PCR in any samples except one PBMC of a control subject. The nested double PCR, which is a more sensitive method for VZV DNA detection, was applied to the same samples. The viral DNA was detected in every PBMC of the controls, and in 2 (16.7%) PBMC at 1 week and in 6 (50%) PBMC at 4 weeks after vaccination in the 12 vaccinees with seroconversion. In 3 of 4 vaccinees who were seropositive before vaccination, VZV DNA was detected in PBMC at 1 or 4 weeks after vaccination. The three vaccinees showed a booster immunization with a significant increase in antibody titers. In contrast, no VZV DNA could be detected in any throat swabs of all the vaccinees nor in PBMC of the vaccinees who did not seroconvert.
采用聚合酶链反应（PCR）和巢式双重PCR对20名接种水痘疫苗的健康儿童进行水痘-带状疱疹病毒（VZV）DNA检测。在接种疫苗前、接种后1周和4周同时采集3次外周血单个核细胞（PBMC）和咽拭子样本进行PCR分析。还从12名患水痘的健康儿童急性期的PBMC中各采集1份样本作为阳性对照。除1名对照受试者的1份PBMC外，首次PCR在任何样本中均未发现VZV DNA。将对VZV DNA检测更敏感的巢式双重PCR应用于相同样本。在对照组的每份PBMC中均检测到病毒DNA，在12名血清阳转的接种者中，接种后1周的2份（16.7%）PBMC和接种后4周的6份（50%）PBMC中检测到病毒DNA。在4名接种前血清阳性的接种者中，有3名在接种后1周或4周的PBMC中检测到VZV DNA。这三名接种者出现了加强免疫，抗体滴度显著升高。相比之下，在所有接种者的任何咽拭子中以及未发生血清阳转的接种者的PBMC中均未检测到VZV DNA。
