Buch J P, Havlovec S K
Department of Surgery, University of Connecticut Health Center, Farmington 06030-3955.
J Urol. 1994 Mar;151(3):619-21. doi: 10.1016/s0022-5347(17)35031-0.
During a 6-month period 59 semen specimens were subjected to the direct immunobead test and 12 were positive (15% or more of sperm bound by IgA or IgG to the head, mid piece or principal piece of the tail, for an incidence of 20%). Mean motility and forward progression (scale of 0 to 4) were 47% (range 10 to 80) and 1.7 (range 1 to 3), respectively, in the positive test group, whereas these values were 53% (range 1 to 90) and 2.2 (range 1 to 3), respectively, in the negative test group. Sperm penetration assay was performed on 11 of the 12 direct immunobead test positive specimens. A normal sperm penetration assay score was noted in 5 of the 11 specimens (46%), a subfertile score in 4 (36%) and an infertile score (no penetrations) in 2 (18%). The incidence of scores in these 3 subcategories of fertility status in the direct immunobead test positive group did not differ significantly from distribution of scores from all 87 sperm penetration assays performed at our laboratory during the same period: normal--39 of 87 (45%), subfertile--35 (40%) and infertile--13 (15%). Furthermore, there was no pathognomonic binding pattern for those antibody positive patients with subfertile or infertile scores. In conclusion, sperm antibody positivity is typically independent of egg penetration ability, there is no pathognomonic antibody binding pattern for poor egg penetration and there is a tendency for antibody positive sperm to have a lower percentage of motility and forward progression.
在6个月的时间里，对59份精液标本进行了直接免疫珠试验，其中12份呈阳性（IgA或IgG与精子头部、中段或尾部主段结合的精子占15%或更多，发生率为20%）。阳性试验组的平均活力和向前运动能力（0至4级）分别为47%（范围10%至80%）和1.7（范围1至3），而阴性试验组的这些值分别为53%（范围1%至90%）和2.2（范围1至3）。对12份直接免疫珠试验阳性标本中的11份进行了精子穿透试验。11份标本中有5份（46%）的精子穿透试验评分正常，4份（36%）为亚生育评分，2份（18%）为不育评分（无穿透）。直接免疫珠试验阳性组这3种生育状态亚类评分的发生率与同期在我们实验室进行的所有87次精子穿透试验的评分分布无显著差异：正常——87份中的39份（45%），亚生育——35份（40%），不育——13份（15%）。此外，对于那些亚生育或不育评分的抗体阳性患者，没有特征性的结合模式。总之，精子抗体阳性通常与卵子穿透能力无关，对于卵子穿透不良没有特征性的抗体结合模式，并且抗体阳性的精子活力和向前运动能力百分比有降低的趋势。
