鉴定两个新型牛淋巴细胞抗原分化簇的单克隆抗体研究：研讨会簇（WC）6和7。
Studies of monoclonal antibodies identifying two novel bovine lymphocyte antigen differentiation clusters: workshop clusters (WC) 6 and 7.
作者信息
Parsons K R, Bembridge G, Sopp P, Howard C J
机构信息
AFRC Institute for Animal Health, Compton, Nr Newbury, UK.
出版信息
Vet Immunol Immunopathol. 1993 Nov;39(1-3):187-92. doi: 10.1016/0165-2427(93)90180-c.
Analysis of flow cytometric staining of 50 target cells resulted in five monoclonal antibodies (mAbs) forming two adjacent clusters called temporary cluster (TC) 2. The five mAbs gave similar immunostaining of frozen sections, but flow cytometric analysis and the M(r) of molecules indicated that they comprised two distinct specificities. Mabs CC98, IL-A114 and IL-A53 all immunoprecipitated a molecule of 210 kDa that preclearing experiments with mAbs to the leukocyte common antigen (CD45) showed did not recognize isoforms within the CD45R family of molecules. The 210 kDa molecule was present on all CD2+ cells and B cells, but not expressed by WC1+ gamma/delta T cells. mAbs TH1A and TH18A precipitated molecules of 69 and 62 kDa which were present on cells expressing CD2, WC3 (B cells) and WC1 (gamma/delta T cells). It is proposed that these five mAbs should be considered as comprising two new bovine lymphocyte differentiation clusters, WC6 (mAbs CC 98, IL-A114 and IL-A53) and WC7 (TH18A and TH1A).
对50个靶细胞进行流式细胞术染色分析，得到5种单克隆抗体（mAb），它们形成了两个相邻的簇，称为临时簇（TC）2。这5种mAb对冰冻切片的免疫染色相似，但流式细胞术分析和分子的相对分子质量（M(r)）表明它们包含两种不同的特异性。mAb CC98、IL-A114和IL-A53均免疫沉淀出一个210 kDa的分子，用针对白细胞共同抗原（CD45）的mAb进行的预清除实验表明，该分子不识别CD45R分子家族中的异构体。210 kDa的分子存在于所有CD2⁺细胞和B细胞上，但WC1⁺γ/δ T细胞不表达。mAb TH1A和TH18A沉淀出69 kDa和62 kDa的分子，这些分子存在于表达CD2、WC3（B细胞）和WC1（γ/δ T细胞）的细胞上。建议将这5种mAb视为包含两个新的牛淋巴细胞分化簇，WC6（mAb CC 98、IL-A114和IL-A53）和WC7（TH18A和TH1A）。
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