Dutia B M, Ross A J, Hopkins J
Department of Veterinary Pathology, University of Edinburgh, UK.
Vet Immunol Immunopathol. 1993 Nov;39(1-3):193-9. doi: 10.1016/0165-2427(93)90181-3.
The WC6 antibovine monoclonal antibodies (mAbs) CC98, IL-A114 and IL-A53 were investigated for reactivity in sheep by (fluorescence activated cell sorting) FACS analysis, immunoprecipitation and immunohistology. The mAbs behave identically by all criteria although IL-A114 reacts more weakly than the other mAbs. This probably reflects limited cross-species reactivity. The mAbs stain < 30% of lymphocytes from blood, efferent and afferent lymph and the majority of afferent lymph dendritic cells. They also weakly stain granulocytes. They precipitate molecules of apparent molecular weight 220 kD and 180-190 kD. Sequential immunoprecipitation shows that CC98 antigen is not related to CD45. Immunohistology indicates staining of B cell areas and macrophages in Peyer's patch and lamina propria. The data show that these monoclonal antibodies react with a molecule distinct from OvCD45R.
通过荧光激活细胞分选（FACS）分析、免疫沉淀和免疫组织学研究了WC6抗牛单克隆抗体（mAb）CC98、IL-A114和IL-A53在绵羊中的反应性。尽管IL-A114的反应比其他单克隆抗体弱，但所有标准下这些单克隆抗体的表现相同。这可能反映了有限的跨物种反应性。这些单克隆抗体对来自血液、输出和输入淋巴的淋巴细胞以及大多数输入淋巴树突状细胞的染色率<30%。它们对粒细胞的染色也较弱。它们沉淀出表观分子量为220 kD和180 - 190 kD的分子。连续免疫沉淀表明CC98抗原与CD45无关。免疫组织学显示派尔集合淋巴结和固有层中B细胞区域和巨噬细胞有染色。数据表明这些单克隆抗体与一种不同于OvCD45R的分子发生反应。
