Hilgert I, Cikánek D, Kristofová H, Karesová R, Navrátil M
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Hybridoma. 1993 Apr;12(2):215-20. doi: 10.1089/hyb.1993.12.215.
Monoclonal antibodies (MAbs) to plum pox virus (PPV) were obtained after immunization of BALB/c mice with purified PPV-W isolate. Spleen cells from a mouse showing a high serum titer were used for fusion with Sp2/0-Ag14 myeloma cells. Culture supernatants were screened for specific antibody production against PPV-W isolate using indirect ELISA. A total of six stable hybridoma lines producing MAbs of IgG class were obtained. All four PPV isolates tested (W, A, D and M) can be distinguished by these MAbs. Two highly efficient antibodies were chosen for practical purposes, and their applicability in PPV diagnostics has been studied since 1989 in parallel with polyclonal antibodies and commercial kits (Boehringer, Bioreba). The experiments have proven comparable sensitivity with a detection limit ranging between 10 and 50 ng of virus per ml sample. In routine diagnostics of plums, peaches and apricots our MAbs ranked at least as high as the commercial kits.
用纯化的李痘病毒（PPV）-W分离株免疫BALB/c小鼠后，获得了针对李痘病毒（PPV）的单克隆抗体（MAbs）。将一只血清滴度高的小鼠的脾细胞与Sp2/0-Ag14骨髓瘤细胞进行融合。使用间接ELISA筛选培养上清液中针对PPV-W分离株的特异性抗体产生情况。共获得了六个产生IgG类单克隆抗体的稳定杂交瘤细胞系。所测试的所有四种PPV分离株（W、A、D和M）都可以通过这些单克隆抗体区分开来。出于实际目的选择了两种高效抗体，自1989年以来，已将它们与多克隆抗体和商业试剂盒（Boehringer，Bioreba）并行研究其在PPV诊断中的适用性。实验证明其具有可比的灵敏度，检测限为每毫升样品10至50纳克病毒。在李子、桃子和杏子的常规诊断中，我们的单克隆抗体至少与商业试剂盒表现相当。
