Haynes D R, Rogers S D, Hay S, Pearcy M J, Howie D W
Department of Orthopaedic Surgery and Trauma, Royal Adelaide Hospital, Australia.
J Bone Joint Surg Am. 1993 Jun;75(6):825-34. doi: 10.2106/00004623-199306000-00004.
We investigated the relationship between the toxic effects of metal wear particles and their ability to stimulate the release of inflammatory mediators implicated in bone resorption. In vitro studies were carried out with use of rat peritoneal macrophages, which were exposed to either cobalt-chromium-alloy or titanium-aluminum-vanadium particles, milled from the metal components of hip prostheses. The particles were in the size-range of, and at concentrations similar to, those found in the tissues surrounding failed prostheses in humans. The titanium-aluminum-vanadium particles showed little toxicity even at high concentrations, while the cobalt-chromium particles were very toxic. The titanium-aluminum-vanadium particles induced significantly more release of prostaglandin E2 than did the cobalt-chromium particles, and this was true for a wide range of concentrations. Exposure to titanium-aluminum-vanadium increased the release of prostaglandin E2, interleukin-1, tumor necrosis factor, and interleukin-6. In contrast, exposure to cobalt-chromium particles was associated with a decreased release of prostaglandin E2 and interleukin-6, and it had little effect on the release of interleukin-1 and tumor necrosis factor.
我们研究了金属磨损颗粒的毒性作用与其刺激参与骨吸收的炎症介质释放能力之间的关系。使用大鼠腹膜巨噬细胞进行了体外研究，这些巨噬细胞暴露于从髋关节假体金属部件研磨而来的钴铬合金颗粒或钛铝钒颗粒。这些颗粒的大小范围和浓度与人类假体失效周围组织中发现的相似。即使在高浓度下，钛铝钒颗粒也几乎没有毒性，而钴铬颗粒则具有很强的毒性。在很宽的浓度范围内，钛铝钒颗粒诱导的前列腺素E2释放量明显多于钴铬颗粒。暴露于钛铝钒会增加前列腺素E2、白细胞介素-1、肿瘤坏死因子和白细胞介素-6的释放。相比之下，暴露于钴铬颗粒会导致前列腺素E2和白细胞介素-6的释放减少，并且对白细胞介素-1和肿瘤坏死因子的释放影响很小。
