Jantzen E, Sonesson A, Tangen T, Eng J
Statens Institutt for Folkehelse, Oslo, Norway.
J Clin Microbiol. 1993 Jun;31(6):1413-9. doi: 10.1128/jcm.31.6.1413-1419.1993.
Twenty-nine species (76 strains) of members of the genus Legionella were analyzed for their cellular hydroxylated fatty acids (OH-FAs). The individual patterns were unusually complex and included both monohydroxylated and dihydroxylated chains of unbranched or branched (iso and anteiso) types. Comparison of the strain profiles by SIMCA (Soft Independent Modelling of Class Analogy) principal component analysis revealed four main groups. Group 1 included Legionella pneumophila plus L. israelensis strains, and group 2 included L. micdadei and L. maceacherneii strains. These two closely related groups were characterized by the occurrence of di-OH-FAs and differed mainly in the amounts of 3-OH-a21:0, 3-OH-n21:0, 3-OH-n22:0, and 3-OH-a23:0. Group 3 (13 species) was distinguished by i14:0 at less than 3%, 3-OH-3-OH-n14:0 at greater than 5%, 3-OH-n15:0 at greater than 2%, and minute amounts of OH-FAs with chains longer than 21:0. Group 4 (12 species) was heterogeneous. Its main characteristics were the presence of 3-OH-n12:0 and 3-OH-n13:0, 3-OH-i14:0 at greater than 5%, as well as significant amounts of 3-OH-a21:0 and 3-OH-n21:0. The groupings obtained by OH-FA profiles were found to reflect DNA-DNA homology groupings reasonably well, and the profiles appear to be useful for differentiation of Legionella species.
对军团菌属的29个种（76株）进行了细胞羟基化脂肪酸（OH-FAs）分析。个体模式异常复杂，包括直链或支链（异链和anteiso链）类型的单羟基化和二羟基化链。通过SIMCA（类分析的软独立建模）主成分分析对菌株谱进行比较，发现了四个主要组。第1组包括嗜肺军团菌和以色列军团菌菌株，第2组包括米克戴德军团菌和马凯内军团菌菌株。这两个密切相关的组的特征是存在二羟基化脂肪酸，主要区别在于3-OH-a21:0、3-OH-n21:0、3-OH-n22:0和3-OH-a23:0的含量。第3组（13个种）的特征是i14:0含量低于3%，3-OH-3-OH-n14:0含量高于5%，3-OH-n15:0含量高于2%，以及链长大于21:0的微量OH-FAs。第组4（12个种）具有异质性。其主要特征是存在3-OH-n12:0和3-OH-n13:0，3-OH-i14:0含量高于5%，以及大量的3-OH-a21:0和3-OH-n21:0。发现通过OH-FA谱获得的分组能够较好地反映DNA-DNA同源性分组，并且这些谱似乎可用于军团菌属物种的鉴别。
