低pH值下免疫复合物解离后人免疫缺陷病毒抗原血症检测增加。
Increased detection of human immunodeficiency virus antigenemia after dissociation of immune complexes at low pH.
作者信息
Pokriefka R A, Manzor O, Markowitz N P, Saravolatz L D, Kvale P, Donovan R M
机构信息
Department of Internal Medicine, Henry Ford Hospital, Detroit, Michigan 48202.
出版信息
J Clin Microbiol. 1993 Jun;31(6):1656-8. doi: 10.1128/jcm.31.6.1656-1658.1993.
Abstract
This study compared the number of patients with detectable human immunodeficiency virus (HIV) antigenemia after immune complex (IC) dissociation by established methods using either 0.5 NCl or 1.5 M glycine buffer. Without IC dissociation, HIV antigen was detected in 43% of patients. After dissociation, the HCl method detected only an additional 7% of patients (P = 0.09), while the glycine method detected an additional 34% (P < 0.001). However, care must be taken in setting the threshold of the standards, and confirmatory neutralization assays should be performed to ensure specificity of HIV antigen enzyme immunoassay after IC dissociation.
摘要
本研究比较了采用既定方法，即使用0.5N盐酸或1.5M甘氨酸缓冲液进行免疫复合物（IC）解离后，可检测到人类免疫缺陷病毒（HIV）抗原血症的患者数量。在不解离IC的情况下，43%的患者检测到HIV抗原。解离后，盐酸法仅额外检测到7%的患者（P = 0.09），而甘氨酸法额外检测到34%的患者（P < 0.001）。然而，在设定标准阈值时必须谨慎，并且应进行确证性中和试验，以确保IC解离后HIV抗原酶免疫测定的特异性。
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