Pokriefka R A, Manzor O, Markowitz N P, Saravolatz L D, Kvale P, Donovan R M
Department of Internal Medicine, Henry Ford Hospital, Detroit, Michigan 48202.
J Clin Microbiol. 1993 Jun;31(6):1656-8. doi: 10.1128/jcm.31.6.1656-1658.1993.
This study compared the number of patients with detectable human immunodeficiency virus (HIV) antigenemia after immune complex (IC) dissociation by established methods using either 0.5 NCl or 1.5 M glycine buffer. Without IC dissociation, HIV antigen was detected in 43% of patients. After dissociation, the HCl method detected only an additional 7% of patients (P = 0.09), while the glycine method detected an additional 34% (P < 0.001). However, care must be taken in setting the threshold of the standards, and confirmatory neutralization assays should be performed to ensure specificity of HIV antigen enzyme immunoassay after IC dissociation.
本研究比较了采用既定方法,即使用0.5N盐酸或1.5M甘氨酸缓冲液进行免疫复合物(IC)解离后,可检测到人类免疫缺陷病毒(HIV)抗原血症的患者数量。在不解离IC的情况下,43%的患者检测到HIV抗原。解离后,盐酸法仅额外检测到7%的患者(P = 0.09),而甘氨酸法额外检测到34%的患者(P < 0.001)。然而,在设定标准阈值时必须谨慎,并且应进行确证性中和试验,以确保IC解离后HIV抗原酶免疫测定的特异性。