Klimov A N, Gurevich V S, Nikiforova A A, Shatilina L V, Kuzmin A A, Plavinsky S L, Teryukova N P
Department of Biochemistry, Institute for Experimental Medicine, St. Petersburg, Russia.
Atherosclerosis. 1993 Apr;100(1):13-8. doi: 10.1016/0021-9150(93)90063-z.
The present study consists of experimental and clinical investigations. It was shown that a single intravenous injection of a large dose of human HDL3 (200 mg protein) to rabbits with induced hypercholesterolemia (plasma cholesterol 500-700 mg/dl) was accompanied by a significant elevation of plasma HDL and led to a decrease (P < 0.05) of conjugated dienes and trienes by 20-30% after 6 h. Conjugated dienes remained stable for 24 h after HDL administration. In the clinical investigations a weak but statistically significant negative correlation (r = 0.262; P = 0.006) between HDL cholesterol and the content of conjugated dienes in the plasma of a total group of healthy subjects and patients with coronary heart disease (CHD) was found. The data allowed us to conclude that, in addition to other antioxidative systems, HDL also take part in the protection of plasma lipids from peroxidation.
本研究包括实验和临床调查。结果表明，给诱导性高胆固醇血症（血浆胆固醇500 - 700mg/dl）的兔子单次静脉注射大剂量人HDL3（200mg蛋白质）后，血浆HDL显著升高，并在6小时后使共轭二烯和三烯减少（P < 0.05）20 - 30%。HDL给药后共轭二烯在24小时内保持稳定。在临床调查中，发现健康受试者和冠心病（CHD）患者的总组血浆中，HDL胆固醇与共轭二烯含量之间存在微弱但具有统计学意义的负相关（r = 0.262；P = 0.006）。这些数据使我们得出结论，除其他抗氧化系统外，HDL也参与保护血浆脂质免受过氧化。
