Raynaud E, Audran M, Pagès J C, Fédou C, Brun J F, Chanal J L, Orsetti A
Service d'Exploration Physiologique des Hormones et des Métabolisme, Hôpital Lapeyronie, Montpellier, France.
Clin Endocrinol (Oxf). 1993 Apr;38(4):353-9. doi: 10.1111/j.1365-2265.1993.tb00514.x.
Exogenous testosterone administration is classically detected by measuring the ratio of testosterone to epitestosterone in urine. Athletes are considered to be positive for drug abuse if the urinary testosterone to epitestosterone ratio is greater than 6. We aimed at investigating the urinary excretion of testosterone and epitestosterone during pubertal development.
We performed a cross-sectional study of 141 normal male subjects between ages 8 and 26 years.
We studied 141 subjects: 32 at stage 1 of Tanner, 27 at stage 2, 30 at stage 3, 25 at stage 4 and 27 at stage 5.
Subjects performed a 24-hour urine collection. Urinary testosterone and epitestosterone were measured by gas chromatography-mass spectrometry with selected ion monitoring.
Urinary testosterone was 20.5 +/- 1.7 nmol/24 h (mean +/- SEM) at stage 1, 49 +/- 2.9 at stage 2, 98.8 +/- 3.4 at stage 3, 371.8 +/- 21.8 at stage 4 and 403.4 +/- 16.1 nmol/24h at stage 5. Urinary epitestosterone was 13.1 +/- 1.5 nmol/24h at stage 1, 29.1 +/- 3.3 at stage 2, 48.3 +/- 3.7 at stage 3, 156.3 +/- 14.8 at stage 4 and 221.1 +/- 18.6 nmol/24h at stage 5. The urinary excretions of both steroids increased significantly during puberty and were highly correlated with chronological age (P < 0.001). Comparison of the correlation slopes (P < 0.001) showed that the urinary profiles of testosterone and epitestosterone are not parallel during pubertal development. Two subjects presented a testosterone to epitestosterone ratio above 6, corresponding to a low urinary concentration of epitestosterone, without pathological explanation.
Testosterone and epitestosterone do not present the same urinary profiles throughout puberty. Marked increases of the testosterone to epitestosterone ratio can be observed at this period and may interfere with doping tests.
外源性睾酮的使用通常通过测量尿液中睾酮与表睾酮的比值来检测。如果尿睾酮与表睾酮比值大于6，则运动员被视为药物滥用阳性。我们旨在研究青春期发育过程中睾酮和表睾酮的尿排泄情况。
我们对141名年龄在8至26岁之间的正常男性受试者进行了横断面研究。
我们研究了141名受试者：坦纳1期32名，2期27名，3期30名，4期25名，5期27名。
受试者进行24小时尿液收集。尿睾酮和表睾酮通过气相色谱 - 质谱联用选择离子监测法进行测量。
尿睾酮在1期为20.5±1.7 nmol/24小时（平均值±标准误），2期为49±2.9，3期为98.8±3.4，4期为371.8±21.8，5期为403.4±16.1 nmol/24小时。尿表睾酮在1期为13.1±1.5 nmol/24小时，2期为29.1±3.3，3期为48.3±3.7，4期为156.3±14.8，5期为221.1±18.6 nmol/24小时。两种类固醇的尿排泄量在青春期均显著增加，且与实际年龄高度相关（P < 0.001）。相关性斜率比较（P < 0.001）表明，青春期发育过程中睾酮和表睾酮的尿谱不平行。两名受试者的睾酮与表睾酮比值高于6，对应表睾酮尿浓度较低，且无病理学解释。
在整个青春期，睾酮和表睾酮的尿谱不同。在此期间可观察到睾酮与表睾酮比值显著增加，这可能会干扰兴奋剂检测。
