Balaghi M, Wagner C
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37212.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):1184-90. doi: 10.1006/bbrc.1993.1750.
Folate deficiency produces a marked decrease in the ratio of S-adenosylmethionine to S-adenosylhomocysteine. This ratio regulates the activity of many cellular methylation reactions including that of DNA. We used bacterial CpG methylase to evaluate the effect of folate deficiency on methylation of DNA from livers of weanling rats fed an amino acid-defined folate deficient diet. After 4 weeks, liver DNA from deficient animals was undermethylated compared with DNA from pair-fed control animals.
叶酸缺乏会导致S-腺苷甲硫氨酸与S-腺苷同型半胱氨酸的比例显著降低。该比例调节许多细胞甲基化反应的活性，包括DNA的甲基化反应。我们使用细菌CpG甲基转移酶来评估叶酸缺乏对喂食氨基酸限定的叶酸缺乏饮食的断奶大鼠肝脏DNA甲基化的影响。4周后，与配对喂食的对照动物的DNA相比，缺乏叶酸动物的肝脏DNA甲基化不足。
