Fujiskai J, Fujimoto K, Oohara A, Sakata T, Hirano M, Ohyama T, Iwakiri R, Yamaguchi M
Department of Internal Medicine, Saga Medical School, Japan.
Dig Dis Sci. 1993 Jul;38(7):1195-200. doi: 10.1007/BF01296067.
To examine the roles of histamine and diamine oxidase in the intestine after ischemia-reperfusion, we measured histamine content, diamine oxidase activity, and ornithine decarboxylase activity in rat intestinal mucosa 6 hr following various periods of ischemia. In addition, mortality rates of rats after various periods of ischemia were observed. The superior mesenteric artery was occluded for 15, 30, or 60 min. Ornithine decarboxylase activity increased in the 15-, 30-, and 60-min ischemic groups compared to the sham-operated control group. In the prolonged ischemic group (60-min ischemia), both histamine concentration and diamine oxidase activity in the mucosa decreased, contributing to an increase in circulating histamine. In the 60-min ischemic group, the mortality rate of rats was 25%, which was significantly larger than the control groups. Pretreatment with aminoguanidine, which suppressed diamine oxidase activity, increased the mortality rate. These results indicate that histamine released from the intestinal mucosa has a harmful effect on rats, and diamine oxidase activity plays an important role when the small intestine is subjected to prolonged period of ischemia.
为研究组胺和二胺氧化酶在肠缺血再灌注后的作用，我们测定了大鼠肠黏膜在经历不同时长缺血6小时后的组胺含量、二胺氧化酶活性和鸟氨酸脱羧酶活性。此外，还观察了大鼠在经历不同时长缺血后的死亡率。肠系膜上动脉分别阻断15、30或60分钟。与假手术对照组相比，15分钟、30分钟和60分钟缺血组的鸟氨酸脱羧酶活性均升高。在长时间缺血组（60分钟缺血）中，黏膜中的组胺浓度和二胺氧化酶活性均降低，导致循环组胺增加。在60分钟缺血组中，大鼠死亡率为25%，显著高于对照组。用氨基胍预处理抑制二胺氧化酶活性后，死亡率增加。这些结果表明，从肠黏膜释放的组胺对大鼠具有有害作用，并且在小肠遭受长时间缺血时，二胺氧化酶活性发挥重要作用。
