Kusche J, Lorenz W, Stahlknecht C D, Richter H, Hesterberg R, Schmal A, Hinterlang E, Weber D, Ohmann C
Gastroenterology. 1981 May;80(5 pt 1):980-7.
It has been suggested that histamine contributes to lethal circulatory collapse after acute superior mesenteric artery occlusion. The activity of the histamine inactivating enzyme diamine oxidase, the release of the amine, and the effect of histamine receptor antagonists was therefore studied in rabbits. The main results were: (a) Diamine oxidase activity decreased by 60% after intestinal ischemia and reperfusion. A monoexponential dose-response relationship was found between the specific diamine oxidase inhibitor aminoguanidine and reduced survival time. (b) Plasma histamine levels in the right atrium rose only slightly after ischemia, but considerably during reperfusion of the gut, and remained high for at least 20 min. In sham-operated animals the plasma histamine concentration was unchanged throughout the experiment. The histamine content in the intestinal wall did not fall significantly at any time after mesenteric artery occlusion and reperfusion. (c) The aminoguanidine-induced reduction in survival time was completely reversed by pre-treatment of the animals with the H1-receptor antagonist dimethylpyridine and the H2-receptor antagonist cimetidine. This study provides strong evidence for the protective role of intestinal diamine oxidase in intestinal ischemia.
有人提出，组胺会导致急性肠系膜上动脉闭塞后出现致命性循环衰竭。因此，对家兔体内组胺灭活酶二胺氧化酶的活性、胺的释放以及组胺受体拮抗剂的作用进行了研究。主要结果如下：(a) 肠缺血再灌注后，二胺氧化酶活性下降了60%。在特定的二胺氧化酶抑制剂氨基胍与缩短的存活时间之间发现了单指数剂量反应关系。(b) 右心房血浆组胺水平在缺血后仅略有升高，但在肠道再灌注期间显著升高，并至少持续20分钟保持在高位。在假手术动物中，整个实验过程中血浆组胺浓度没有变化。肠系膜动脉闭塞再灌注后，肠壁中的组胺含量在任何时候都没有显著下降。(c) 用H1受体拮抗剂二甲吡啶和H2受体拮抗剂西咪替丁对动物进行预处理，可完全逆转氨基胍引起的存活时间缩短。这项研究为肠道二胺氧化酶在肠道缺血中的保护作用提供了有力证据。
