Marmor M F, Dalal R
Department of Ophthalmology, Stanford University School of Medicine, California 94305-5308.
Invest Ophthalmol Vis Sci. 1993 Jul;34(8):2570-5.
Pressure-induced ocular ischemia is a frequent model for the investigation of the mechanisms and therapy of retinal ischemic damage. It is important to know whether the tissue damage in such experiments is uniform or irregular.
We reviewed histologic features of Dutch rabbit eyes after 60-80 min of pressure-induced ischemia. The eyes were enucleated 4 hr, 1 day, or 1 wk after circulation was restored, at which times the electroretinogram b-wave was moderately reduced.
Light microscopy showed an irregular distribution of damage involving all retinal layers and retinal pigment epithelium. Some regions of damage (or preservation) were several millimeters wide; others were as small as a few cell widths. Correlation with electroretinogram reduction in individual eyes was difficult.
These results show that pressure-induced ischemic damage in the rabbit, sufficient to reduce the electroretinogram, has a patchy and irregular effect on retina and retinal pigment epithelium. Erroneous judgments may be made about ischemic damage, or therapeutic intervention, if only small or selected regions of retina are examined histologically.
压力诱导性眼部缺血是研究视网膜缺血性损伤机制及治疗方法常用的模型。了解此类实验中组织损伤是均匀的还是不规则的很重要。
我们观察了荷兰兔眼在压力诱导性缺血60 - 80分钟后的组织学特征。在恢复血液循环后4小时、1天或1周摘除眼球，此时视网膜电图b波中度降低。
光学显微镜检查显示损伤分布不规则，累及所有视网膜层和视网膜色素上皮。一些损伤（或保存）区域宽达数毫米；其他区域则小至几个细胞宽度。难以将个体眼中视网膜电图降低情况与之关联。
这些结果表明，足以降低视网膜电图的兔压力诱导性缺血损伤对视网膜和视网膜色素上皮具有斑片状且不规则的影响。如果仅对视网膜的小区域或选定区域进行组织学检查，可能会对缺血损伤或治疗干预做出错误判断。
