Hilton D A, Variend S, Pringle J H
Department of Pathology, Leicester Royal Infirmary, U.K.
J Pathol. 1993 May;170(1):45-51. doi: 10.1002/path.1711700108.
This is a combined study using in situ hybridization and the polymerase chain reaction to investigate the presence of Coxsackie virus RNA in formalin-fixed tissue from cases of childhood myocarditis. Of the ten cases studied, two were positive by both methods. The virus RNA was predominantly located in areas showing an inflammatory cell infiltrate and myofibre necrosis. These findings suggest that direct lytic infection of myocytes by virus is responsible for myocarditis in these cases, rather than an autoimmune process, which has been suggested previously. The findings in one case, where the virus showed a marked sub-endocardial distribution, may have implications for the aetiology of endocardial fibroelastosis by confirming a viral tropism for this location. The techniques used in this study are easily repeatable and can be directly applied to look for viruses in a number of other diseases where a viral aetiology is suspected.
这是一项联合研究，运用原位杂交和聚合酶链反应来调查儿童心肌炎病例福尔马林固定组织中柯萨奇病毒RNA的存在情况。在所研究的10例病例中，有2例两种方法检测均呈阳性。病毒RNA主要位于有炎性细胞浸润和肌纤维坏死的区域。这些发现表明，在这些病例中，病毒对心肌细胞的直接溶解性感染是心肌炎的病因，而非先前曾提出的自身免疫过程。在1例病例中，病毒显示出明显的心内膜下分布，这一发现通过证实病毒对该部位的嗜性，可能对心内膜弹力纤维增生症的病因学有启示意义。本研究中使用的技术易于重复，可直接应用于在许多怀疑有病毒病因的其他疾病中寻找病毒。
