Aymard J P, Botte C, Contal P, Janot C, Streiff F
Centre Régional de Transfusion Sanguine de Nancy, Vandceuvre-les-Nancy, France.
Pathol Biol (Paris). 1993 Feb;41(2):149-53.
Between March and November 1991, prevalence of hepatitis C antibodies was evaluated in 60,960 blood donors from the North-East of France. Using a second generation ELISA, 424 donors (0.69%) were reactive, with no significant difference between males (0.69%) and females (0.70%). Among these 424 donors, respectively 137 (32.3%), 86 (20.3%) and 201 (47.4%) were reactive, indeterminate or nonreactive by a second generation RIBA (RIBA-2) (Recombinant Immunoblot Assay). Donors with a high ELISA ratio (> or = 4) were significantly more likely to have a reactive RIBA-2. Of the 1906 donors with anti-HBc positivity (3.12%), 44 had a reactive ELISA; of these, respectively 27, 12 and 5 had a reactive, indeterminate and nonreactive RIBA. Of the 1201 donors (1.97%) with increased serum ALAT (alanine-amino-transferase) levels (> or = 2N), 42 had a positive ELISA; of these, respectively 35, 2 and 5 had a reactive, indeterminate and nonreactive RIBA. Of the 54 donors with both indirect markers, nine had a reactive ELISA; the same nine donors had a reactive RIBA. These data show that donors with both surrogate markers and a reactive ELISA are very likely to have a positive RIBA. Seventy-seven (18.16%) of the 424 donors with a reactive ELISA had at least one surrogate marker; 67 of these donors (30.04%) were among the 223 donors with a reactive ELISA and a reactive or indeterminate RIBA.
1991年3月至11月期间，对法国东北部的60960名献血者进行了丙型肝炎抗体患病率评估。使用第二代酶联免疫吸附测定法（ELISA），424名献血者（0.69%）检测呈阳性反应，男性（0.69%）和女性（0.70%）之间无显著差异。在这424名献血者中，分别有137名（32.3%）、86名（20.3%）和201名（47.4%）通过第二代重组免疫印迹法（RIBA-2）检测呈阳性反应、结果不确定或呈阴性反应。ELISA比值高（≥4）的献血者更有可能RIBA-2检测呈阳性反应。在1906名抗-HBc呈阳性（3.12%）的献血者中，44名ELISA检测呈阳性反应；其中，分别有27名、12名和5名RIBA检测呈阳性反应、结果不确定和呈阴性反应。在1201名血清丙氨酸转氨酶（ALAT）水平升高（≥2N）（1.97%）的献血者中，42名ELISA检测呈阳性；其中，分别有35名、2名和5名RIBA检测呈阳性反应、结果不确定和呈阴性反应。在同时具有两种间接标志物的54名献血者中，9名ELISA检测呈阳性反应；这9名献血者RIBA检测均呈阳性反应。这些数据表明，同时具有替代标志物且ELISA检测呈阳性反应的献血者很可能RIBA检测呈阳性。424名ELISA检测呈阳性反应的献血者中有77名（18.16%）至少有一种替代标志物；其中67名献血者（30.04%）在223名ELISA检测呈阳性反应且RIBA检测呈阳性反应或结果不确定的献血者中。
