Robberecht P, Cremer M, Christophe J
Gastroenterology. 1977 Mar;72(3):417-20.
The pancreatic secretion of 6 normal human volunteers was collected by endoscopic cannulation of the main pancreatic duct. The appearance of newly synthesized proteins was monitored at 1-min intervals after labeling with [75Se]methionine. The minimum transit time of these proteins from their site of synthesis to the acinar lumen was 36 +/- 8 min. Stimulation of protein secretion by a rapid intravenous injection of caerulein (40 ng per kg), 1 hr after [75Se]methionine administration, greatly decreased (by 73% on an average) the specific radioactivity of the discharged proteins. These data support the concept of a functional heterogeneity of proteins secreted by the human pancreas.
通过内镜插管主胰管收集6名正常人类志愿者的胰腺分泌物。用[75Se]蛋氨酸标记后，每隔1分钟监测新合成蛋白质的出现情况。这些蛋白质从合成部位到腺泡腔的最短转运时间为36±8分钟。在给予[75Se]蛋氨酸1小时后，通过快速静脉注射雨蛙素（每公斤40纳克）刺激蛋白质分泌，大大降低了（平均降低73%）排出蛋白质的比放射性。这些数据支持了人类胰腺分泌的蛋白质存在功能异质性的概念。
