Llobet J M, Colomina M T, Sirvent J J, Domingo J L, Corbella J
Laboratory of Toxicology and Biochemistry, School of Medicine, Rovira i Virgili University, Reus, Spain.
Toxicology. 1993 Jun 11;80(2-3):199-206. doi: 10.1016/0300-483x(93)90181-q.
The reproductive toxicity of vanadium was studied in mice. Male Swiss mice were exposed to sodium metavanadate at doses of 0, 20, 40, 60, and 80 mg/kg per day given in the drinking water for 64 days. To evaluate the fertility of the vanadium-treated animals, males were mated with untreated females for 4 days. A significant decrease in the pregnancy rate was observed at 60 and 80 mg/kg per day of sodium. metavanadate. However, metavanadate did not reduce fertility in male mice at 20 and 40 mg/kg per day. Reproductive toxicity was measured by sperm count, sperm motility, organ weights, and histologic evaluation of the testes. Decreased body and epididymis weight was only observed in the 80 mg/kg per day group, while testicular weights were not altered by the treatment with all doses used. Sperm count was significantly decreased at 40, 60, and 80 mg/kg per day, but the sperm motility was unaffected. Histopathological examination revealed that the testes were normal and that the epididymis of treated male mice contained normal appearing sperm. The no observed adverse effect level (NOAEL) was 40 mg/kg per day. Consequently, vanadium would not cause any adverse effect on fertility or testicular function in male mice at the concentrations usually ingested by humans through the diet and drinking water.
研究了钒对小鼠的生殖毒性。将雄性瑞士小鼠暴露于偏钒酸钠，通过饮用水给予剂量为0、20、40、60和80毫克/千克，持续64天。为评估经钒处理动物的生育能力，将雄性小鼠与未处理的雌性小鼠交配4天。观察到每天给予60和80毫克/千克偏钒酸钠时，怀孕率显著下降。然而，每天给予20和40毫克/千克偏钒酸钠时，未降低雄性小鼠的生育能力。通过精子计数、精子活力、器官重量和睾丸组织学评估来测量生殖毒性。仅在每天80毫克/千克组观察到体重和附睾重量下降，而所有使用剂量的处理均未改变睾丸重量。每天给予40、60和80毫克/千克时，精子计数显著下降，但精子活力未受影响。组织病理学检查显示睾丸正常，经处理的雄性小鼠附睾中含有外观正常的精子。未观察到有害作用水平（NOAEL）为每天40毫克/千克。因此，按照人类通常通过饮食和饮用水摄入的浓度，钒不会对雄性小鼠的生育能力或睾丸功能造成任何有害影响。
